活化素A对MPP^＋所诱导的PC12细胞损伤的保护作用

目的观察重组人活化素A(rhAcT)对MPP+所诱导的PC12细胞损伤的保护作用.方法将MMP+或活化素A,L-deprenyl加入体外培养的PC12细胞,用四甲基偶氮唑盐(MTT)法检测细胞活力的变化;用免疫细胞化学法和RT-PCR评价细胞的酪氨酸羟化酶和bcl-2蛋白及mRNA含量的变化,用脱氧核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡的变化,比较各组的差异.结果预先给予rhAcT和L-deprenyl的两组细胞活力明显高于MPP+损害组,酪氨酸羟化酶和bcl-2的蛋白及mRNA表达强于损害组,同时两组的凋亡细胞明显减少,rhAcT和L-deprenyl两组间无显著差异.结论rhAcT和L-deprenyl通过上调bcl-2的表达,抑制凋亡的发生,而对MMP+所诱导的PC12细胞的损伤具有保护作用.

Protective Effect of Activin A to the Injury of PC12 Cell Induced by MPP+

Aim:To observe the protective effect of recombinant human activin A (rhAcT) and L-deprenyl to the PC12 cell injury induced by MPP +.Methods:MPP +, activin A or L-deprenyl was added to the culture media of PC12 cells, to assay the cell viability with MTT method; to evaluate the change of content of protein and mRNA of tyrosine hydroxylase and bcl-2 with immunocytochemistry and RT-PCR techniques respectively,also, the number of apoptotic cells was investigated by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL) method,thus the difference between the groups was compared.Results:In the group of activin A or L-deprenyl,the cell viability was higher than that treated only with MPP +,the content of protein and mRNA of tyrosine hydroxylase and bcl-2 also greater than the injuried group and the number of apoptotic cells was decreased significantly.No observable difference existed between activin A and L-deprenyl group.Conclusion:rhAct A and L-deprenyl may have a protective effect to the injury of PC12 cells induced by MPP + by upregulating bcl-2 and inhibiting apoptosis.