| Akt激酶抑制剂MK-2206诱导U2OS人骨肉瘤细胞凋亡与自噬 |
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| 引用本文： | 王雪莹,李照梅,周运生,郭文莉,王凤泽. Akt激酶抑制剂MK-2206诱导U2OS人骨肉瘤细胞凋亡与自噬[J]. 中国病理生理杂志, 2014, 30(9): 1580-1583. DOI: 10.3969/j.issn.1000-4718.2014.09.007 |
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| 作者姓名： | 王雪莹 李照梅 周运生 郭文莉 王凤泽 |
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| 作者单位： | 1泰山医学院生物科学学院，山东 泰安 271016； 2泰安市中心医院放疗中心，山东 泰安 271000；3淄博市博山区疾病预防控制中心，山东 淄博 255200 |
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| 基金项目： | 国家自然科学基金资助项目（No.81272683）；山东省自然科学基金资助项目（No.ZR2011HQ034） |
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| 摘 要： | 目的：研究Akt抑制剂MK-2206对U2OS细胞凋亡和自噬的影响。方法：采用MTT法检测MK-2206对U2OS细胞活力的影响，DNA片段末端标记试剂盒检测细胞凋亡的变化，免疫印迹法检测细胞内蛋白的表达，用LC3-II的表达量用来确定细胞的自噬水平。结果：MK-2206剂量依赖性地降低了U2OS细胞的活力；MK-2206能够促进caspase-9、caspase-3和PARP的活化切割而诱导U2OS细胞发生凋亡；MK-2206给药后可促进细胞内LC3-II的表达，氯喹阻断自噬后明显增强了MK-2206对U2OS细胞活力的抑制作用。结论：Akt 抑制剂MK-2206能够诱导U2OS细胞发生凋亡和自噬；抑制自噬可促进MK-2206对U2OS细胞的毒性。
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| 关 键 词： | MK-2206 细胞凋亡 细胞自噬 U2OS细胞 |
| 收稿时间： | 2014-04-01 |
MK-2206, an inhibitor of Akt,induced cell apoptosis and autophagy in U2OS cells |
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| WANG Xue-ying,LI Zhao-mei,ZHOU Yun-sheng,GUO Wen-li,WANG Feng-ze. MK-2206, an inhibitor of Akt,induced cell apoptosis and autophagy in U2OS cells[J]. Chinese Journal of Pathophysiology, 2014, 30(9): 1580-1583. DOI: 10.3969/j.issn.1000-4718.2014.09.007 |
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| Authors: | WANG Xue-ying LI Zhao-mei ZHOU Yun-sheng GUO Wen-li WANG Feng-ze |
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| Affiliation: | 1School of Biological Science, Taishan Medical College, Taian 271016, China; 2Department of Radiation Oncology, Central Hospital of Taian, Taian 271000, China; 3The Center for Disease Control and Prevention of Boshan District, Zibo 255200, China. |
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| Abstract: | AIM：To observe the effect of MK-2206, an inhibitor of Akt, on the cell apoptosis and autophagy of U2OS cells. METHODS：The cell viability was detected by MTT assay. The cell apoptosis was analyzed by TdT-mediated dUTP nick end labeling assay. The expression of LC3-II was examined by Western blotting. RESULTS：MK-2206 inhibited the cell viability in a dose-dependent manner. MK-2206 induced the cell apoptosis via activation of caspase-3, caspase-9 and PARP. MK-2206 treatment substantially induced the U2OS cell autophagy by increasing in the levels of LC3-II. Blockage of autophagy using chloroquine magnified MK-2206-induced cell death in U2OS cells. CONCLUSION：The Akt inhibitor MK-2206 induces cell apoptosis and autophagy. Blocking autophagy magnifies MK-2206-induced the inhibition of the viability in U2OS cells. |
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| Keywords: | MK-2206 Apoptosis Cell autophagy U2OS cells |
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