大豆异黄酮减轻反式脂肪酸诱导的大鼠动脉粥样硬化

目的观察大豆异黄酮减轻反式脂肪酸诱导的大鼠动脉粥样硬化的作用。方法将大鼠随机分为普通饲料组、反式脂肪酸组、大豆异黄酮组和反式脂肪酸+大豆异黄酮组,用试剂盒分别测量血中TG、HDL、LDL、IL-6和TNF-α的含量;培养牛主动脉内皮细胞,分为对照组、反式脂肪酸组和反式脂肪酸+大豆异黄酮组,Western blot法检测牛主动脉内皮细胞内NF-κB的磷酸化,同时检测细胞间黏附因子-1(ICAM-1)与血管细胞黏附因子(VCAM-1)蛋白表达。结果与普通饲料组比较,反式脂肪酸组血TG、LDL、IL-6和TNF-α明显升高(P0.05),HDL显著降低(P0.05)。大豆异黄酮干预后,血中TG、LDL、IL-6和TNF-α均降低(P0.01),HDL显著升高(P0.01)。大豆异黄酮能降低反式脂肪酸诱导的NF-κB磷酸化(P0.01);与对照组比较,大豆异黄酮能使反式脂肪酸诱导的ICAM-1和VCAM-1的表达降低(P0.01)。结论大豆异黄酮可减轻反式脂肪酸诱导的大鼠动脉粥样硬化作用。

Soybean isoflavone relieving the atherosclerosis of rats induced by trans fatty acids

Objective To observe the effects of soybean isoflavone relieving atherosclerosis of rats induced by trans fatty acids. Methods The rats were randomly divided into normal diet group、trans fatty acids group、soybean isoflavone group、trans fatty acids+soybean isoflavone group. Research kit was used to test the amounts of TG、HDL、LDL and IL-6、TNF-ɑ in rats’ blood.Bovine aortic endothelial cells were cultured to be divided into control group、trans fatty acids group and trans fatty acids group+soybean isoflavone group.Western blot was performed to test the NF-?B phosphorylation,as well as to determine ICAM-1 and VCAM-1 protein contents which bovine aortic endothelial cells contained.Results Compared with normal diet group,the amounts of TG、LDL、IL-6、TNF-ɑ increased evidently(P<0.05), whereas the amounts of HDL decreased evidently (P<0.05) in the rats’blood of trans fatty acids group.After the soybean isoflavone intervened,the amounts of TG、LDL、IL-6、TNF-ɑ all decreased(P<0.01),meanwhile the amounts of HDL rose largely(P<0.01). Soybean isoflavone could lower the NF-κB phosphorylation induced by trans fatty acids(P<0.01). The high expression of ICAM-1 and VCAM-1 induced by trans fatty acids can be decreased by soybean isoflavone when comparing with those in the control group(P<0.01). Conclusions Soybean isoflavone has a role in relieving the rats’atherosclerosis reduced by trans fatty acids.