慢病毒介导的CCN1基因对大鼠骨髓间充质干细胞

 目的：探讨外源性CCN1对大鼠骨髓间充质干细胞（BMSCs）生长和迁移的作用。方法：构建带有绿色荧光蛋白的大鼠CCN1慢病毒载体（Lenti-GFP-CCN1）并感染大鼠BMSCs，筛选最适感染复数（MOI）；实验分为空白组、感染Lenti-GFP组和感染Lenti-GFP-CCN1组，倒置荧光显微镜下观察BMSCs感染后荧光表达情况；MTT法检测细胞存活率，划痕愈合实验检测细胞迁移作用。结果：与空白组和阴性对照组相比，Lenti-GFP-CCN1感染大鼠BMSCs后，细胞的存活率未见明显差异；感染组细胞划痕愈合实验的愈合宽度/原宽度值与空白组及阴性对照组相比差异显著（P<0.05）。结论：外源性CCN1对正常大鼠BMSCs存活率无影响，可促进BMSCs迁移。

Lentivirus mediated CCN1 gene on growth and migration of rat bone marrow mesenchymal stem cells

AIM：To investigate the role of cysteine-rich 61 (Cyr61/CNN1) in proliferation and migration of bone marrow mesenchymal stem cells (BMSCs). METHODS：The lentiviral vector carrying CCN1 (Lenti-GFP-CCN1) was constructed and then transfected into the rat BMSCs. The cells were divided into non-transfection group, transfection group (transfected with Lenti-GFP-CCN1) and negative control group (Lenti-GFP). The fluorescence intensity of the transfected BMSCs was observed under inverted fluorescence microscope. The effects of CCN1 on the proliferation and migration of BMSCs were detected by MTT assay and scratch wound healing assay. RESULTS：The proliferation of BMSCs transfected with Lenti-GFP CCN1 had no significant difference compared with negative control group and control group. The width/thickness ratio of migrated BMSCs in wound healing was significantly higher in Lenti-GFP-CCN1 group than that in negative control group and control group (P<0.05). CONCLUSION：Exogenous CCN1 promotes the migration of BMSCs.