HMGB1、TLR4和NF-B在子痫前期患者胎盘组织及血浆中升高

目的探讨高迁移率族蛋白(HMGB1)、TOLL受体4(TLR4)和NF-B信号通路在子痫前期中的相关作用。方法轻度子痫前期患者10例、重度子痫前期患者20例和同期正常妊娠者30例。免疫组织化学(SP法)检测胎盘中HMGB1、TLR4和NF-κB P65蛋白的表达变化及在组织中的定性、定位;用ELISA法检测血清中HMGB1、TLR4和NF-κB P65蛋白浓度。结果子痫前期患者胎盘中HMGB1、TLR4、和NF-κB P65蛋白表达高于正常对照组(P0.05);轻度和重度子痫前期患者间无差异。子痫前期患者血清中HMGB1、TLR4和NF-κB P65的含量较正常组明显升高(P0.05);且重度子痫前期患者血清中HMGB1、TLR4、和NF-κB P65蛋白表达高于轻度子痫前期患者(P0.05)。结论 HMGB1、TLR4及NF-κB P65蛋白表达水平在子痫前期患者胎盘及血清中显著升高,可能参与了子痫的发病过程。

Increase of HMGB1, TLR4 and NF-kB in placenta tissue and serum in patients with preeclampsia

Objective To evaluate the expression and discussion on high mobility group protein ( HMGB1)/toll like receptor 4 (TLR4) and NF-?B possible role in the signaling pathway in preeclampsia. Methods 10 cases of patients with mild preeclampsia(MP), 20 patients with severe preeclampsia(SP) and 30 cases of normal pregnancy as a research object in the same period.To navigate the qualitative expression of the HMGB1,TLR4,NF-?Bp65 protein in placenta tissue using Immunohistochemical staining.Levels of HMGB1,TLR4,NF-?Bp65 in blood serum were measured by ELISA. Result (1) Immunohistochemistry showed that the expression of HMGB1,TLR4,NF-?Bp65 were stronger than that of control group in the placental of preeclampsia（P＜0.05);But HMGB1,TLR4,NF-?Bp65 in placenta of patients with severe preeclampsia and mild preeclampsia comparison of the differences is no statistically significant（P＞0.05).(2) Levels of HMGB1、TLR4、NF-?Bp65 in women with preeclampsia significantly higher than control group( P<0.05); Levels of HMGB1、TLR4、NF-?Bp65 in women with severe preeclampsia higher than with mild preeclampsia (P＜0.05).Conclusion HMGB1,TLR4 and NF-?Bp65 were up-expressed in the placenta tissue and serum in patients with preeclampsia, which indicated that HMGB1,TLR4 and NF-?Bp65 may play an important role in the development of preeclampsia.