p110δ突变失活的ApcMin/+结直肠癌癌前病变小鼠模型的建立

 目的：建立p110δ突变失活的ApcMin/+结直肠癌癌前病变小鼠模型，为研究p110δ在小鼠结直肠癌癌前病变中的作用提供实验模型。方法：将C57BL/6J背景的ApcMin/+结直肠癌癌前病变小鼠与p110δ突变失活小鼠（p110δD910A/D910A）进行杂交建系，通过PCR技术鉴定子代小鼠基因型，获得p110δ突变失活的ApcMin/+小鼠。对适龄小鼠进行肠道取材，亚甲蓝染色后，观察肠道结构，对腺瘤和微腺瘤进行统计。肠道组织进行石蜡包埋、切片，做HE染色进行进一步观察。结果：获得p110δ突变失活的ApcMin/+杂交鼠（ApcMin/+；p110δD910A/D910A）并得以稳定传代。ApcMin/+；p110δD910A/D910A杂交小鼠的肠道组织中，腺瘤数目和体积比ApcMin/+结直肠癌癌前病变小鼠均有减少。结论：成功建立p110δ突变失活的ApcMin/+结直肠癌癌前病变小鼠模型，并得到小鼠肠道肿瘤的初步表型，为进一步研究p110δ在肠道肿瘤发生发展中的作用提供重要的工具动物。

Establishment of a transgenic heterozygous mouse model of ApcMin/+ precancerosis of colorectal cancer with p110δ mutation

AIM：To establish a transgenic heterozygous mouse model of precancerous lesions of colorectal cancer with p110δ mutation in the C57BL/6J background for serving the studies on colorectal cancer research mediated by p110δ. METHODS：The transgenic heterozygous mice were generated by crossing in p110δD910A/D910A mouse and ApcMin/+ mouse, and the genotype was detected by PCR. Compared with ApcMin/+ mice, transgenic heterozygous mice (ApcMin/+; p110δD910A/D910A)were counted, and the number and size of intestine polyps were analyzed after methylene blue staining. The intestinal tissue structure was assessed by HE staining. RESULTS：The transgenic heterozygous mouse model of precancerous lesions of colorectal cancer with p110δ mutation was established. The number and size of polyps in the transgenic heterozygous mice were declined. CONCLUSION：A transgenic heterozygous mouse model of precancerous lesions of colorectal cancer with p110δ mutation was successfully established. The initial phenotype of intestinal tumors in transgenic mice was observed. This model will greatly contribute to the related research of colorectal cancer in mice.