| 高迁移率族蛋白B1对大鼠局灶脑缺血/再灌注后大脑皮质梗死周围区神经干细胞增殖的影响 |
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| 引用本文： | 李满,罗勇,李圆,孙麟. 高迁移率族蛋白B1对大鼠局灶脑缺血/再灌注后大脑皮质梗死周围区神经干细胞增殖的影响[J]. 中国病理生理杂志, 2014, 30(9): 1616-1621. DOI: 10.3969/j.issn.1000-4718.2014.09.013 |
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| 作者姓名： | 李满 罗勇 李圆 孙麟 |
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| 作者单位： | 1山西医科大学第二医院神经内科，山西 太原 030001； 2重庆医科大学附属第一医院神经内科，重庆市神经病学重点实验室，重庆 400016； 3山西医科大学基础医学院，山西 太原 030001； 4山西医学科学院, 山西大医院骨科，山西 太原 030032 |
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| 基金项目： | 国家自然科学基金资助项目（No. 30470606）；重庆市卫生局中医药科研项目（No. 2003-B-16） |
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| 摘 要： | 目的：探讨高迁移率族蛋白B1（HMGB1）对局灶脑缺血/再灌注大脑皮质梗死周围区神经干细胞增殖的影响。方法：选取雄性SD大鼠48只，分为假手术组、模型组、RNA干扰组和阴性干扰组。采用线栓法制备大鼠局灶脑缺血/再灌注模型，缺血1 h再灌注7 d时观测。用携带大鼠HMGB1 shRNA的慢病毒载体介导RNA干扰抑制脑内HMGB1的表达，通过免疫荧光双标记HMGB1/GFAP和Western blotting法检测RNA干扰效果。通过免疫荧光双标记5-溴脱氧尿嘧啶（BrdU）/神经巢蛋白（nestin）检测大脑皮质梗死周围区神经干细胞的增殖。结果：与假手术比较，再灌注7 d时，模型组大脑皮质梗死周围区HMGB1蛋白表达明显升高（P<0.05）；与模型组比较，RNA干扰有效抑制HMGB1蛋白的表达（P<0.05）。与假手术组比较，模型组的BrdU/nestin双标记细胞明显增多（P<0.05）；与模型组比较，RNA干扰组的BrdU/nestin双标记细胞明显减少（P<0.05）。结论：局灶脑缺血/再灌注后大脑皮质梗死周围区HMGB1蛋白表达水平升高可促进该部位的神经干细胞增殖。
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| 关 键 词： | 局灶脑缺血/再灌注 高迁移率簇蛋白B1 神经干细胞 RNA干扰 慢病毒 |
| 收稿时间： | 2013-11-08 |
Influence of high-mobility group box 1 on proliferation of neural stem cell in pre-infarction cortex of focal cerebral ischemia/reperfusion model rats |
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| LI Man,LUO Yong,LI Yuan,SUN Lin. Influence of high-mobility group box 1 on proliferation of neural stem cell in pre-infarction cortex of focal cerebral ischemia/reperfusion model rats[J]. Chinese Journal of Pathophysiology, 2014, 30(9): 1616-1621. DOI: 10.3969/j.issn.1000-4718.2014.09.013 |
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| Authors: | LI Man LUO Yong LI Yuan SUN Lin |
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| Affiliation: | 1Department of Neurology, The Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China; 2Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016, China; 3Basic Medical College of Shanxi Medical University, Taiyuan 030001, China; 4Department of Orthopedics, Shanxi Academy of Medical Sciences, Shanxi Dayi Hospital, Taiyuan 030032, China. |
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| Abstract: | AIM：To investigate the influence of high-mobility group box 1 (HMGB1) on the proliferation of neural stem cells in peri-infarction cortex of focal cerebral ischemia/reperfusion model rats. METHODS：Male SD rats (n=48) were randomly divided into sham group, ischemia/reperfusion (I/R) group, RNA interference group and negative interference group. The rat middle cerebral artery was blocked to establish focal cerebral I/R model (ischemia for 1 h and reperfusion for 7 d). Lentivirus vector of HMGB1 shRNA was used to suppress the HMGB1 protein expression in the rat brain. The effect of RNA interference was evaluated by the methods of double-immunofluorescence labeling of HMGB1/GFAP and Western blotting. The proliferation of neural stem cells in the peri-infarction cortex was assessed by double labeling of BrdU/nestin. RESULTS：The protein expression of HMGB1 in I/R group was much higher than those in sham group (P<0.05). RNA interference effectively inhibited the HMGB1 expression (P<0.05). Double labeled BrdU/nestin positive cells in I/R group were more than that in sham group (P<0.05). The double labeled BrdU/nestin positive cells were significantly decreased in RNA interference group (P<0.05). CONCLUSION：Focal cerebral ischemia/reperfusion injury promotes the proliferation of neural stem cells in peri-infarction cortex by increasing HMGB1 protein level. |
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| Keywords: | Focal cerebral ischemia/reperfusion High-mobility group box 1 Neural stem cells RNA interference Lentivirus |
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