黄芩苷诱导人肝癌HepG-2细胞凋亡作用观察 |
| |
引用本文: | 梁慧敏,时小燕,和振坤,徐庆杰.黄芩苷诱导人肝癌HepG-2细胞凋亡作用观察[J].实用肝脏病杂志,2012,15(4):343-345. |
| |
作者姓名: | 梁慧敏 时小燕 和振坤 徐庆杰 |
| |
作者单位: | 河南大学淮河医院感染病科,河南省开封市,475001 |
| |
摘 要: | 目的研究黄芩苷对人肝癌HepG-2细胞凋亡的影响,并探讨其作用机制。方法以不同浓度的黄芩苷与人肝癌HepG-2细胞共同培养,采用MTT法测定细胞增殖抑制率;采用Hoechst33258/PI染色法在荧光显微镜下观察凋亡细胞形态学变化;采用TUNEL法检测细胞凋亡率;采用Western blot法检测细胞凋亡相关蛋白Caspase-9、Caspase-3和Bcl-2表达的变化。结果在25~100μg/ml的药物浓度作用下,细胞增殖被抑制。药物浓度在50μg/ml下作用48h,细胞抑制率达50.63%,药物浓度在75μg/ml下作用48h细胞,抑制率达77.62%。抑制率呈浓度和时间依赖性;药物浓度在50μg/ml时作用48h,可见HepG-2细胞皱缩、细胞核碎裂成碎片,呈现典型的凋亡改变;随着黄芩苷作用浓度的增加,细胞凋亡率增高(P<0.05);随药物浓度的增加,Caspase-9和Caspase-3蛋白表达量呈增加趋势,而Bcl-2表达减少。结论黄芩苷能通过诱导细胞凋亡进而抑制人肝癌细胞增殖,其诱导凋亡机制可能与线粒体通路有关。
|
关 键 词: | 肝癌 HepG2细胞 黄芩苷 细胞凋亡 |
Inhibition of apoptosis of HepG-2 cells by bacalin in vitro |
| |
Institution: | Liang Huimin,Shi Xiaoyan,He Zhengkun et al.Department of Infectious Diseases,Huaihe Hospital,Affiliated to Henan University,Kaifeng 475001,China |
| |
Abstract: | Objective To investigate the inhibition of apoptosis of HepG-2 cells by bacalin in vitro.Methods Human hepatocellular carcinoma cells,HepG-2 were cultured with different concentrations of baicalin and the inhibitory rate of cell proliferation was calculated with MTT assay;Morphology changes were detected by Hoechst33258/PI double fluorescence coloration;The apoptosis rate was detected by TUNEL;the Caspase-9,Caspase-3 and Bcl-2 protein expression were detected by Western blot.Results The cell proliferation was inhibited by baicalin at concentration of 25 to 100μg/ml;the inhibitory rate in 48h was 50.63% at 50μg/ml and 77.62% at 75μg/ml,and the inhibitory effect was in the concentration and time-dependent manner;there was a typical apoptosis at 50μg/ml in 48h,showing cell shrinkage and fragmentation of cell nucleus;the caspase-9 and caspase-3 expression increased and Bcl-2 decreased as the concentration of baicalin increased.Conclusion Baicalin can inhibit proliferation of HepG2 cells by inducing apoptosis and the mechanisms might be related to the mitochondrial pathway. |
| |
Keywords: | Hepatoma HepG2 cells Baicalin Apoptosis |
本文献已被 CNKI 维普 万方数据 等数据库收录! |
|