鼠尾草酸对肥胖性脂肪性肝炎大鼠血清生化指标和肝组织学变化的影响

目的探讨鼠尾草酸（CA）对肥胖性大鼠脂肪性肝炎的影响。方法 随机将28只SD大鼠分为对照组（n=8）和实验组（n=20），分别喂以普通饲料和高脂饲料，10周后，将实验组大鼠随机分配到模型组（n=10）和CA组（n=10），后者以cA200mg．kg^-1．d^-1体重灌胃，共6周。16周后，检测肝功能、血脂、胰岛素、SOD、MDA和脂肪细胞因子水平变化，并观察肝组织学变化。结果在16周末，对照组和模型组大鼠体重分别为449．5±47．4g对531．8±40．4g，内脏脂肪重为4．51±1．63g对6．37±1．66g，胰岛素抵抗指数（HOMA—IR）为0．30±0．15对0．65±0．34，ALT为36±18U／L对144±80U／L，AST为132±29U／L对218±64U／L，MDA为5．93±1．14mmol／ml对18．89±7．04mmol／ml，SOD为24．61±0．43U／ml对22．91±0．93U／ml，脂联素为3．58±0．70μg／ml对4．44±0．49μg／ml，瘦素为475．78±143．76pg／ml对651．86±185．92pg／ml，抵抗素为2．71±0．79ng／ml对5．23±2．67ng／ml（P〈0．01）；肝组织病理学检查显示模型组动物肝内呈重度脂肪变性；与模型组比，CA组大鼠上述各项指标均有不同程度的改善。结论CA对高脂饮食诱导的肥胖及脂肪性肝炎有很好的治疗效果。CA可能是抑制了内脏脂肪积聚，调节脂代谢，改善胰岛素抵抗，对抗氧化应激和脂质过氧化损伤。

Effects of carnosic acid on the changes of serum biochemical parameters and liver histology in obese rats with non-alcoholic steatohepatitis

Objective To explore the effect of carnosic acid （CA）on high-fat diet （HFD）-induced obese non-alcoholic steatohepatitis （NASH）in rats. Methods Twenty-eight male Sprague-Dawley rats were randomly divided into control group （n=8） and experimental group（n=20）. After 10 weeks,rats fed with HFD were assigned to model group （n=10） and treatment group （n=lO）,which were both fed with HFD and treated with intragastrie administration of either NS or CA （200mg.kg^-1.d^-1） for 6 weeks. Results The rats in model group developed abdominal obesity and NASH in liver histopathology;the body weights in control and model groups were 449.5±47.4 g vs 531.8±40.4 g,visceral fat weight：4.51±1.63g vs 6.37±l.66g,TC：l.58±0.14 mmol/L vs 3.21±0.71mmol/L, ALT：36±18U/L vs 144±80U/L,AST：132±29U/L vs 218±64U/L,MDA：5.93±1.14nmol/ml vs 18.89±7.04nmol/ml, HOMA-IR：0.30±O.15 vs 0.65±0.34 and SOD：24.6±0.43U/ml vs 22.91±0.93U/ml （P〈0.01）;Compared with those in the model group, improvement of all above indexes was found in CA-treated group. Conclusion Carnosic acid has satisfactory therapeutic effects on obesity and steatohepatitis induced with high-fat diet. Inhibiting the accumulation of visceral fat,regulating lipid metabolism,improving insulin resistance,resisting oxidative stress and lipid peroxidation damage may be part of its therapeutic mechanisms.