桂皮醛对NIH3T3细胞增殖和细胞周期的影响

目的观察肉桂主要成分桂皮醛对NIH3T3细胞增殖的影响。方法采用MTT法观察不同浓度桂皮醛对NIH3T3细胞增殖的影响,并采用流式细胞仪检测桂皮醛对NIH3T3细胞周期的影响。结果桂皮醛作用NIH3T3细胞48 h后,细胞增殖速度较对照组明显增加,增殖率为15%(P<0.01)。流式细胞仪检测结果显示,桂皮醛作用24 h后NIH3T3细胞S期比例上升了3%(P<0.05),G2/M期比例无明显升高,细胞增殖指数(PrI)增加了3.5%(P<0.01)。结论桂皮醛可促进NIH3T3的增殖,这种促增殖作用可能是通过调节细胞周期使更多细胞进入S期而实现的。

Effect of cinnamyl aldehyde on proliferation and cycle of NIH3T3 cells

Objective To observe the effect of cinnamyl aldehyde(main principle of Cortex einnamomi) on the proliferation of NIH3T3 cells.Methods The effect of cinnamyl aldehyde in different concentrations on the proliferation of NIH3T3 cells was observed by using MTT assay,and on NIH3T3 cell cycle,by using flow cytometer.Result The cell proliferation rate(15%) increased obviously 48 hours after cinnamyl aldehyde acting on NIH3T3 cells(P<0.01).The result of flow cytometer assay showed that the percentage of NIH3T3 cells in S phase was increased by 3%(P<0.05),the percentage in G_2/M phase was not increased and proliferate index(PrI) was increased by 3.5%(P<0.01) 24 hours after the action of cinnamyl aldehyde.Conclusion Cinnamyl aldehyde can improve the(proliferation) of NIH3T3 cells,which may be related to that cinnamyl aldehyde can promote more cells(into) S phase through regulating the cell cycle.