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An epitope shared by central nervous system myelin and peripheral blood macrophages
Authors:Kari Stefansson  Anthony T Reder  Jack P Antel
Institution:1. Reproductive Medicine Center, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, PR China;2. Harvard-MGH Center for Reproductive Medicine and Reproductive Endocrine Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA;3. Clinical Medicine Research Center of Prenatal Diagnosis and Birth Health in Hubei Province, Wuhan, 430071, Hubei, PR China;4. Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, PR China;1. Division of Pharmacology, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy;2. Department of Biomedical Sciences, Section of Neuroscience and Clinical Pharmacology, University of Cagliari, Cagliari, Italy;3. Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Belvaux, Luxembourg;4. “C. Golgi” Women Health Center, University of Brescia, Brescia, Italy;1. King?s College London, Institute of Psychiatry, Psychology and Neuroscience, Department of Psychosis Studies, PO63, De Crespigny Park, London SE5 8AF, UK;2. King?s College London, Institute of Psychiatry, Psychology and Neuroscience, Department of Basic and Clinical Neuroscience, The James Black Centre, 125 Coldharbour Lane, London SE5 9NU, UK;3. King?s College London, Institute of Psychiatry, Psychology and Neuroscience, Department of Neuroimaging, PO89, De Crespigny Park, London SE5 8AF, UK;1. University of California, Davis, Dept. of Plant Sciences, One shields Ave., Davis, CA 95616-8683, USA;2. University of California, Davis, Dept. of Land, Air and Water Resources, One shields Ave., Davis, CA 95616-8683, USA;3. University of California Cooperative Extension, 1031 S. Mt. Vernon Ave., Bakersfield, CA 93307, USA;1. Center for Advanced Biomaterials for Healthcare@CRIB, Istituto Italiano di Tecnologia (IIT), largo barsanti e matteucci 53, 80125 Naples, Italy;2. Interdisciplinary Research Centre on Biomaterials (CRIB) and Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, University of Naples Federico II, Piazzale Tecchio 80, 80125 Naples, Italy;3. Department of Precision Medicine, University of Campania ‘L. Vanvitelli’, Naples, Italy;4. TRASE S.R.L., viaToledo 265, 80132 Naples, Italy;5. Department of Medical, Surgical, Neurological, Metabolic and Aging Sciences, University of Campania Luigi Vanvitelli, 80138 Naples, Italy
Abstract:Lewis rats were immunized with a homogenate of human spinal cord. Splenocytes from the immunized rats were fused with cells from the SP2/0-Ag14 cell line to form hybrids that were subsequently screened immunohistochemically for secretion of antibodies against myelin. Thirty hybrids secreting anti-myelin antibodies were cloned. One secreted antibody (774) that immunohistochemically stained central nervous system (CNS) myelin but not peripheral nervous system (PNS) myelin also bound to the surface of peripheral blood macrophages. Hence we have identified an epitope that is shared by peripheral blood macrophages and CNS myelin.
Keywords:CNS myelin  Macrophages  Monoclonal antibody  Shared epitope
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