妊娠滋养细胞疾病中EMT相关蛋白CK19和N-cad表达的研究

目的:探讨上皮细胞向间质转化(EMT)相关蛋白细胞角蛋白19(CK19)和神经钙黏蛋白(N-cad)在妊娠滋养细胞疾病发生、发展中的作用。方法:采用免疫组化SP法检测了41例正常早孕绒毛(NP)、31例葡萄胎(HM)、32例妊娠滋养细胞肿瘤(GTN)[26例侵蚀性葡萄胎(IM)+6例绒癌(CCA)]中CK19和N-cad的定位及表达情况。结果:NP组中CK19和N-cad蛋白相对表达均显著高于HM、GTN组(P0.05),HM组显著高于GTN组(P0.05)。CK19与N-cad的表达无相关性(r=0.202,P=0.268)。结论:伴随着滋养细胞恶性程度的升高,CK19和N-cad表达逐渐降低,提示EMT可能与滋养细胞的恶性转化有关。

The expression of epithelial to mesenchymal transition associated protein of CK19 and N-cad in gestational trophoblastic disease

Objective：To explore the role of epithelial to mesenchymal transition （EMT） associated protein of Cytokeratin 19 （CK19） and N-cadherin （N-cad） in oncogenesis, development of gestational trophoblastic disease （ GTD） . Methods：CK19 and N-cad were de-tected Immunohistochemically in paraffin-embedded tissue of 41 cases of normal placenta （ NP） ,31 cases of hydatidiform mole （ HM ） , 32 cases of gestational trophoblastic neoplasia （ GTN ） [ 26 cases of invasive hydatidiform mole （ IM ） and 6 cases of choriocarcinoma （CCA）]. Results：In NP,HM and GTN,the median mean density of CK19 was （7. 62＆#177;5. 54） ＆#215;10-2,（4. 76＆#177;3. 33）＆#215;10-2,（3. 20＆#177;3. 84）＆#215;10-2,respectively,the median mean density of N-cad was （10.6＆#177;8.39）＆#215;10-2,（4.15＆#177;4.19）＆#215;10-2,（2.28＆#177;3.05）＆#215;10-2,respectively. There were significant differences between NP and HM,GTN （P〈0. 05）,and between HM and GTN （ P〈0 . 05 ） . There was no correlation between CK19 and N-cad （ r=0 . 202 , P=0 . 268 ） . Con-clusions：The expressions of CK19 and N-cad decrease with the increase of tropohblast malig-nancy which indicate that CK19 and N-cad may involve in the events of malignant transforma-tion of trophocytes,and EMT may play an important role in the malignant progression of GTD.