| 炒王不留行配方颗粒高效液相指纹图谱研究 |
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| 引用本文： | 陈琳,胡昌江,冯建,周一帆,张彤,高源.炒王不留行配方颗粒高效液相指纹图谱研究[J].亚太传统医药,2013,9(4):16-18. |
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| 作者姓名： | 陈琳 胡昌江 冯建 周一帆 张彤 高源 |
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| 作者单位： | 陈琳 (成都中医药大学,四川成都,611130)； 胡昌江 (成都中医药大学,四川成都,611130)；冯建 (四川新绿色科技发展股份有限公司,四川成都,611930)；周一帆 (成都中医药大学,四川成都,611130)；张彤 (成都中医药大学,四川成都,611130)；高源(成都中医药大学,四川成都,611130)； |
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| 摘 要： | 目的:采用高效液相色谱法建立炒王不留行配方颗粒的指纹图谱,为炒王不留行配方颗粒质量标准提供依据。方法:Boston C18色谱柱(5μm,4.6mm×250mm),流速:1.0mL/min,柱温:30℃,检测波长:280nm,流动相为甲醇(A)-0.3%的磷酸水溶液(B),采用梯度洗脱,梯度洗脱程序为:0～30min(10%～40%A),30～40min(40%A),40～44min(40%～45%A)。结果:建立HPLC指纹图谱共有模式,10批炒王不留行配方颗粒相似度大于0.95。结论:该方法准确、可靠、重复性好,可作为炒王不留行配方颗粒质量控制的较好手段。
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| 关 键 词： | 炒王不留行配方颗粒 指纹图谱 质量控制 高效液相色谱法 |
Research on HPLC Fingerprint of Fried Cowherb seed Formula granules |
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| Abstract: | Objective:To develop a high performance liquid chromatography(HPLC) fingerprint of fried cowherb seed formula granules.Methods:The chromatographic column was Boston C18(5μm,4.6mm×250mm).The mobile phase was methanol and 0.3% phosphoric acid water with gradient elution.Flow rate was 1.0mL/min at 30℃ and detection wavelengh was 280 nm.Results:The common mode of HPLC fingerprint was developed and quality of fried cowherb seed formula granules was similar.Conclusion:This method is accurate and reproducible,which can be used for the quality control of fried cowherb seed formula granules. |
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| Keywords: | Fried Cowherb Seed Formula Granules Fingerprint Quality Control HPLC |
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