| HPLC法测定不同来源山茱萸中马钱苷的含量 |
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| 引用本文： | 袁厚兰,王琳.HPLC法测定不同来源山茱萸中马钱苷的含量[J].亚太传统医药,2013,9(4):23-25. |
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| 作者姓名： | 袁厚兰 王琳 |
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| 作者单位： | 袁厚兰 (泸州医学院附属中医医院,四川泸州,646000)； 王琳 (泸州医学院药学院,四川泸州,646000)； |
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| 摘 要： | 目的:测定不同来源山茱萸中马钱苷的含量。方法:采用反相高效液相色谱法(RP-HPLC)。采用Hypersil ODS色谱柱(5μm,250mm×4.6mm);乙腈-水(15:85)为流动相;检测波长为240nm;流速为1.0mL/min;柱温为25℃。结果:各药材中马钱苷含量分别在0.77%～1.57%。马钱苷在0.02～0.80μg/mL浓度范围内具有良好线性关系(r=0.999 9),平均回收率为99.51%,相对标准偏差(RSD)为0.67%。结论:品种来源不同的山茱萸中马钱苷的含量不同,其中以河南宛西山茱萸中马钱苷含量最高。
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| 关 键 词： | 马钱苷 山茱萸 高效液相色谱法 药物含量测定 |
Determination of Loganin in the Cornus Officinalis from Different Sources by HPLC |
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| Abstract: | Objective:To determinate the content of loganin in the Comus officinalis in differen tsources.Methods:The content of loganin was determined by reversed phase-high performance liquid chromatography.Staionary phase was Hypersil ODS(5μm,250mm×4.6mm),mobile phase was acetonitrile-H2O(15:85,v/v),detection wavelength 240nm,flow rate 1.0mL/min.,column temperature 25℃.Results:The loganin levels in each sample were between 0.77%~1.57%.There was good linearity within the range of 0.02~ 0.80μg/mL for loganin,the average recovery of loganin was 99.51%(RSD=0.67%).Conclusion:There is the content difference of loganin in all samples in different sources,in which the loganin levels in Cornus officinalis from Wanxi Henan was the highest. |
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| Keywords: | Loganin Cornus Officinalis HPLC Content Assaying |
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