| 连接酶链反应(LCR)技术检测男性尿标本的淋病奈瑟菌 |
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| 引用本文： | 田桢干,方筠,张晓航,叶巍. 连接酶链反应(LCR)技术检测男性尿标本的淋病奈瑟菌[J]. 中国艾滋病性病, 2005, 11(6): 436-437 |
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| 作者姓名： | 田桢干 方筠 张晓航 叶巍 |
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| 作者单位： | 上海出入境检验检疫局,上海,200335 |
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| 摘 要： | 目的应用连接酶链反应（LCR）技术检测男性尿标本中的淋病奈瑟菌,初步评价其敏感性和特异性。方法采集受检者晨起或较长时间（2小时以上）不排尿后的首段尿（FVU）标本1131例,利用LCR检测此尿液标本中的淋球菌,针对Cut-off值在灰区以上的标本进行聚合酶链反应（PCR）检测。对LCR和PCR结果相异的标本,用另一LCR试剂复检,参照“扩大的金标准”来确定检测结果。结果LCR的敏感性和特异性分别为100％和99．9％。结论LCR作为一种探针检测技术,是一种既敏感又特异的诊断方法,可避免取尿道标本给患者带来的痛苦,可作为筛检男性淋病奈瑟菌感染的一种非损伤性方法。
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| 关 键 词： | 连接酶链反应 聚合酶链反应 淋病奈瑟菌 |
| 文章编号： | 1672-5662(2005)06-0436-02 |
| 收稿时间： | 2004-10-19 |
| 修稿时间： | 2005-05-19 |
The ligase chain reaction for detection of neisseria gonorrhoeae in male urine samplas |
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| TIAN Zhen-gan, FANG yun, ZHANG Xiao-hang ,et al.. The ligase chain reaction for detection of neisseria gonorrhoeae in male urine samplas[J]. Chinese JOurnal of Aids & STD, 2005, 11(6): 436-437 |
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| Authors: | TIAN Zhen-gan FANG yun ZHANG Xiao-hang et al. |
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| Affiliation: | Shanghai Entry-exit Inspection and Quarantine Bureau, Shanghai 200335, China |
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| Abstract: | Objective To determine the sensitivity and specificity of ligase chain reaction(LCR)for detection of neisseria gonorrhoeae in the male urine.Methods First-void urine(FVU)was examined with LCR plasmid assay in 1131 males and PCR to detect the samples that cut-off value was above equivocal zone.Discordant results were resolved with a second LCR.Test performance was calculated on the basis of an"expanded gold standard".Results The sensitivity and specificity of LCR plasmid assay was 100% and 99.9% respectively.Conclusion LCR is a sensitive and specific diagnostic method for neisseria gonorrhoeae in FVU as a genetic probe;which is a non-invasive diagnostic method for screening neisseria gonorrhoeae infection in male. |
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| Keywords: | Ligase chain reaction PCR Neisseria gonorrhoeae |
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