冠心病患者载脂蛋白A5基因多态性与冠状动脉病变程度的相关性

目的分析冠心病患者载脂蛋白A5基因多态性与冠状动脉病变程度的关系。方法采用聚合酶链反应—限制片长多态性技术分别对260例经冠状动脉造影确诊为冠心病的研究对象载脂蛋白A5基因-1131T>C和c.553G>T多态性位点基因型进行检测;其冠状动脉病变程度由病变支数及Gensini积分表示。结果冠心病患者载脂蛋白A5-1131CC基因型人群和c.553T等位基因携带者血清甘油三酯水平明显高于-1131T等位基因携带者和c.553GG基因型人群(P=0.016和0.008);不同冠状动脉病变支数组间载脂蛋白A5基因型分布和不同基因型间Gensini积分的差异无统计学意义(P>0.05);冠状动脉病变支数和Gensini积分与糖尿病发病率呈显著正相关(r=0.141和0.143,P均<0.05),而与血清高密度脂蛋白胆固醇水平则呈显著负相关(r=-0.129和-0.164,P均<0.05)。结论冠心病患者载脂蛋白A5基因-1131T>C和c.553G>T多态性与其血清甘油三酯水平存在一定的相关性,但与冠状动脉病变程度无关。

The Associated Study of ApolipoproteinA5 Gene Polymorphisms with the Degrees of Coronary Artery Stenosis in Coronary Heart Disease Patients

Aim To investigate the association of apolipoproteinA5(ApoA5) gene polymorphisms of patients with coronary heart disease(CHD)and the degree of coronary artery stenosis. Methods Using polymerase chain reaction-restriction fragmentpolymorphisms of patients with CHD and the degree of length polymorphism methods,the genotypes of 260 patients with CHD confirmed by coronary angiography were analyzed,the levels of coronary artery stenosis were characterized by the numbers of diseased vessels and Gensini scores. Results Serum triglyceride level of the patients with ApoA5-1131CC genotype and c.553T allele carriers were obviously higher than that of the patients with ApoA5 c.553GG genotype and-1131T allele carriers,but the Gensini scores were not significantly different among them,and the distribution for the genotyopes of ApoA5 genetic variants were also not statistically different among variable number of coronary diseased vessels.The number of coronary diseased vessels and Gensini scores had strongly positive correlation with the presence of diabetes(r=0.141 and 0.143,P<0.05),and obviously negative correlation with the level of serum high density lipoprotein cholesterol(r=-0.129 and-0.164,P<0.05). Conclusions ApoA5 gene polymorphisms are remarkably associated with the level of triglyceride,but not the extent of coronary artery stenosis among CHD patients.