蛇毒溶栓素的毒理研究

本文报道溶栓素对动物的急性及亚急性毒性实验,溶血实验,过敏试验和热原检查.给小白鼠静脉注射溶栓素LD_(50)=57u/kg、SLD_(50)=1.54u/kg,肌肉注射400u/kg,观察48小时,未见动物死亡.给猫用5及25u/kg,大白鼠用2、25及50u/kg,每天腹腔注射一次,连续14天(对照组用2%新鲜蛋清液).结果用药后转氨酶、尿素氮、红细胞、白细胞、血小板等各项指标有的上升有的下降,但其均值基本上在正常范围之内.形态学观察正常.病理学检查,肝、肾、心、脑等器官组织有浊肿、空泡样的病变,且与剂量呈平行关系.停药后各器官的病变明显减轻或完全恢复正常.过敏试验、溶血试验及热原检查均为阴性.

STUDIES ON THE TOXICITY ON ANTITHROMBOTIC ENZYMES(ATE) SEPARATED FROM HALY''''S SNAKE VENOM

Antithrombotic enzyme (ATE) is an active component separated from the Agkistrodon halys venom by the chromatographic method.Its acute i.v toxicity was carried out in compliance with the modified Kaeber method.LD_(50) was 57u/kg in mice.Maximal tolerable dose was 400u/kg i.m.A 14 days subacute toxicity test with i.p.antithrombotic enzyme was conducted in 18 cats and 80 rats.Three dose groups, i.e 2u/kg, 25u/kg and 50u/kg(per day), ran parallelly with a 2% albumen solution control.Symptoms and signs, hematology, blood chemistry, necropsy and histopathological examinations were examined at definite intervals.The results reveal that plasma GPT, B.U.N, red blood cell, white blood cell count and platelet count are within normal limits (P>0.05).But, inflammation, hyaline change, cloudy swelling and congestion of the organs including the brain, heart, liver, lungs, muscles, kidneys are also found in the middle and high dose groups.However, after a 14 days recovery period all these morphorlogical changes return to normal.The allergy test, hematolysis test and pysogen test are all negative.