胸腺五肽减轻慢性乙醇摄取小鼠的戒断症状

目的:通过观察胸腺五肽(thymopentin, TP5)对慢性乙醇摄取小鼠觅酒行为和乙醇戒断后焦虑症状的影响,探讨TP5改善乙醇依赖的可能性.方法:依次强迫小鼠饮乙醇溶液:5%(体积分数)1周、10% (体积分数)1周、15%(体积分数)持续4周以建立慢性乙醇摄取模型.给药组连续腹腔注射TP5[0.2 mg/(kg·d), 0.4 mg/(kg·d)]14 d,对照组给予等体积生理盐水,测定给药前后小鼠乙醇(ethanol,E)和水(water,W)的摄取量,以E/(E W)×100%作为其对乙醇选择的指标.用明暗箱和高架十字迷宫评定乙醇戒断后小鼠的焦虑程度.结果:(1)E/(E W)×100%:TP5两剂量组药后值低于药前值.(2)明暗箱试验:TP5两剂量组明暗室间穿梭的次数及在明室内停留的时间药后值明显多于同组药前值和模型对照组药后值.(3)高架十字迷宫试验:TP5两剂量组药后在开臂内停留时间多于药前值和模型对照组药后值,在关臂内停留时间少于药前值.结论:TP5连续腹腔注射14天可减少慢性乙醇摄取小鼠对乙醇的选择,减轻乙醇戒断后的焦虑症状.

Withdrawal symptoms of ethanol are ameliorated by thymopentin in mice

OBJECTIVE: To explore the effect of thymopentin (TP5) on the choice of ethanol and ameliorating withdrawal symptoms (anxiety) of ethanol in mice. METHODS: Mice were administered ethanol (v/v) in schedular fashion: 5% (1 week), 10% (1 week) and 15% (4 weeks), followed with the free choice between ethanol and water. Ethanol/(ethanol+water)x100% [E/(E+W)x100%] was measured as an index of ethanol selection. Light-dark box test and elevated plus maze test were chosen for the assessment of anxiety pre-drug and post-drug. After TP5 [0.2 mg/(kgxd), 0.4 mg/(kgxd)], i.p. or saline (vehicle control), i.p. for 14 days, the procedure was repeated. RESULT: (1) E/(E+W)x100%: the post-drug values of TP5 (0.2 mg/kg, 0.4 mg/kg) were lower significantly than the pre-drug values. (2) Light-dark box test: the post-drug values of number of entries and time spent in the light chamber of TP5 (0.2 mg/kg, 0.4 mg/kg) were more than the pre-drug values themselves and the post-drug value of saline. (3) Elevated plus maze test: the post-drug values of time spent on open arms of TP5 (0.2 mg/kg, 0.4 mg/kg) were more than the pre-drug values themselves and the post-drug value of salineìand the post-drug values of time spent on close arms of TP5 were less than the pre-drug values. CONCLUSION: TP5 could decrease the uptake of ethanol and ameliorate anxious behavior associated with ethanol withdrawal in mice.