自然流产小鼠蜕膜组织中PCNA、Ki-67和survivin表达水平的研究

目的比较正常妊娠和自然流产小鼠模型蜕膜组织中增殖细胞核抗原（PCNA）、细胞增殖标记物Ki-67和凋亡蛋白抑制因子suvivin的表达情况，探讨自然流产的发病机制。方法建立正常妊娠模型CBAXBALB／c和自然流产模型CBAXDBA／2。采用免疫组化SP法测定两组模型孕13d蜕膜细胞PCNA、Ki-67和suvivin的表达。结果与正常妊娠模型相比，自然流产模型蜕膜细胞PCNA的表达显著降低（P〈0．05）；Ki-67和survivin表达降低非常显著（P〈0．01）。结论自然流产小鼠蜕膜细胞PCNA、Ki-67和survivin表达水平低下可能与自然流产的发生有关。

Study on the expressions of PCNA, Ki-67 and survivin in decidua tissue of spontaneous abortion mice

Objective： To investigate the pathogenetic mechanism for spontaneous abortion by comparing the expression level of proliferating cell nuclear antigen （PCNA）, Ki-67 and survivin in decidua tissue of spontaneous abortion with normal pregnancy mice. Methods. The nonabortion-prone （CBA× BALB/c） matings were established as the models of normal pregnancy and the abortion-prone （CBA × DBA/2） matings were used as the models of spontaneous abortion. Immunohistochemistry method（SP） was employed to detect the expressions of PCNA,Ki-67 and survivin in decidua tissue at the day 13 of gestation. Results. Compared with normal pregnant mice, the expression levels of PCNA, Ki-67 and survivin in decidua of spontaneous abortion models were all significantly decreased （P〈0. 05, P〈0.05 and P〈 0.01）. Conclusion： It may be related to spontaneous abortion during early pregnancy that expressions of PCNA, Ki-67 and survivin in decidua were decreased.