Drosophila dSAP18 is a nuclear protein that associates with chromosomes and the nuclear matrix, and interacts with pinin, a protein factor involved in RNA splicing |
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| Authors: | Elisabet Costa Silvia Canudas Ivan Garcia-Bassets Silvia Pérez Irene Fernández Ernest Giralt Fernando Azorín M Lluïsa Espinás |
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| Institution: | (1) Departament de Biologia Molecular i Cellular, Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Científic de Barcelona, Josep Samitier 1-5, 08028 Barcelona, Spain;(2) Servei d’Espectrometria de Masses, SCT, Universitat de Barcelona, Martí i Franqués 1-11, 08028, Barcelona, Spain;(3) Institut de Recerca Biomèdica de Barcelona, Parc Científic de Barcelona, Josep Samitier 1-5, 08028 Barcelona, Spain;(4) Departament de Química Orgánica, Universitat de Barcelona, Martí i Franqués 1-11, 08028 Barcelona, Spain;(5) Present address: Department of Pathology, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA;(6) Present address: Departament of Medicine, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA |
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| Abstract: | SAP18 is a highly conserved protein that was proposed to be involved in multiple cellular processes from autophagy to gene
regulation and mRNA processing. In this paper we show that, in Drosophila, dSAP18 is a predominantly nuclear protein that associates to both chromosomes and the nuclear matrix. dSAP18 becomes nuclear
early during development, at the onset of cellularization, and remains so all through embryo development. dSAP18 is also nuclear
in salivary glands, ovaries and cultured S2 cells. Here we also show that dSAP18 forms a complex with the Drosophila homolog of pinin (dPnn), a protein factor involved in mRNA splicing. dSAP18-dPnn interaction was confirmed in vivo, through co-immunoprecipitation experiments, as well as in vitro, through GST pull-down assays. These results are discussed in the context of the possible functions played by SAP18. |
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| Keywords: | mRNA splicing nuclear matrix pinin SAP18 |
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