| 重组人促红细胞生成素对人脐静脉内皮细胞HoxB2及HoxD3 mRNA表达影响 |
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| 引用本文： | 周乾毅,袁新初,张伟,程桂荣. 重组人促红细胞生成素对人脐静脉内皮细胞HoxB2及HoxD3 mRNA表达影响[J]. 解剖学研究, 2010, 32(5) |
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| 作者姓名： | 周乾毅 袁新初 张伟 程桂荣 |
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| 作者单位： | 武汉科技大学医学院组织学与胚胎学教研室,湖北,武汉,430065 |
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| 基金项目： | 湖北省教育厅重点基金 |
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| 摘 要： | 目的研究重组人促红细胞生成素(rHuEPO)调节体外培养内皮细胞参与血管生成机制。方法从人脐静脉体外分离培养内皮细胞(HUVEC),采用形态学观察及血管内皮细胞Ⅷ因子相关抗原(Ⅷ-R Ag)免疫组织化学方法检测进行内皮细胞鉴定,用逆转录-聚合酶链反应(RT-PCR)技术检测HUVEC同源盒HoxB2及HoxD3基因mRNA表达变化。结果与对照组比较,rHuEPO可明显上调内皮细胞HoxB2及HoxD3 mRNA表达水平,差异有统计学意义(P0.05)。结论 rHuEPO可调节内皮细胞HoxB2及HoxD3 mRNA表达,这种调节作用可能是其促血管生成的主要原因之一。
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| 关 键 词： | 重组人促红细胞生成素 血管内皮细胞 脐静脉 同源盒基因,HoxB2,HoxD3 Ⅷ因子相关抗原 |
Effects of recombinant human erythropoietin on the expressions of homeobox B2 and homeobox D3 gene mRNA in human umbilical vein endothelial cell |
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| ZHOU Qian-Yi,YUAN Xin-Chu,ZHANG Wei,CHENG Gui-rong. Effects of recombinant human erythropoietin on the expressions of homeobox B2 and homeobox D3 gene mRNA in human umbilical vein endothelial cell[J]. Anatomy Research, 2010, 32(5) |
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| Authors: | ZHOU Qian-Yi YUAN Xin-Chu ZHANG Wei CHENG Gui-rong |
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| Abstract: | Objective To study the regulation of recombinant human erythropoietin(rHuEPO)on endothelial cell invloving in mechanism of angiogenesis.Methods The cultured endothelial cells were separated from human umbilical vein and identified by light microscopy and the factor Ⅷ-related antigen.The expression of the factor Ⅷ-related antigen was measured by immunohistochemical method.In addition,The expressions of HoxB2 and HoxD3 mRNA were detected by RT-PCR.Results The expressions of HoxB2 and HoxD3 mRNA in the cultured endothelial cells were up-regulated obviously when compared with those in the control group(P<0.05).Conclusion The rHuEPO could regulate the expressions of HoxB2 and HoxD3 mRNA in the cultured endothelial cells,which may be an important cause for angiogenesis. |
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| Keywords: | Recombinant human erythropoietin Vessel endothelial cell Umbilical vein Homeobox gene,HoxB2,HoxD3 Factor Ⅷ-related antigen |
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