氟铃脲原药大鼠亚慢性毒性实验研究

目的了解氟铃脲原药对大鼠的亚慢性毒性，求出最大无作用剂量。方法将80只sD大鼠随机分为4组，每组20只，分别给氟铃脲原药800、400、200、0mg／（kg·d）灌胃染毒90d，每周称量大鼠体重，90天后检测各项血生化指标，测定脏器重量并计算脏器系数，光学显微镜下对脏器进行病理学检查。结果氟铃脲原药大剂量组800mg／（kg·d）]、中剂量组400mg／（kg·d）]肝组织病理学检查可见形态改变，血清天冬氨酸转氨酶（AST）活力升高。小剂量组200mg／（k·d）]各项指标与对照组比较未见异常。结论氟铃脲原药对大鼠有肝脏毒性，最大无作用剂量为200mg／（kg·d）。

Experimental study on subchronic toxicity of Hexaflumuron in rats

Objective]To study the subchronic toxicity of Hexaflumuron in rats and obtain no observed adverse effect level （ NOAEL}. Methods] A total of 80 SD rats were randomly divided into four groups, with 20 in each group. The rats were gavaged hexaflumu- ron at dose of 800 mg/（ kg·d }, 400 rag/（ kg ·d ）, 200 mg/（ kg·d }, 0 mg/（ kg· d ） for 90 days. Body weight was measured per week. Serum biochemical indicators were determined at the end of the study . Organ weight was measured and organ index was calculated. Pathological examination was performed on organs under an optical microscope. Results]The results showed pathological changes of the liver were observed in the high dose 800 mg/（ kg·d } ] group and medium dose 400 mg/（ kg·d } ] group. Serum activity of aspartate aminotrans ferase（ AST} increased in rats. No significant changes in indicators were found in the low dose group 200 mg/（ kg·d} ]. Conclusion]The Hexaflumuron can induce liver harm, The NOAEL is 200 mg/（kg·d}.