雌激素对大鼠阴道平滑肌细胞RhoA/Rho激酶表达的影响

目的 研究去势及雌激素替代对大鼠阴道平滑肌细胞RhoA/Rho激酶表达的影响.方法 30只10周龄雌性SD大鼠随机均分成:对照4和6周组(C4,C6)、去势4和6周组(OE4,OE6)和去势+雌二醇(E2)干预4和6周组(E4,E6).E4和E6组分别于去势后每周皮下注射E2(250 μg/kg)4和6周,OE4和OE6组注射等量0.9％氯化钠注射液,4和6周时测定血清E2水平及用免疫组化和Western blot检测阴道平滑肌细胞Rock1、Rock2的表达.结果 OF4和OE6组血清E2水平显著低于C4和C6(P＜0.01),OF4和OE6组阴道平滑肌细胞Rock1表达显著高于C4和C6(0.261 ±0.039 vs 0.207 ±0.032;0.270 ±0.037 vs 0.208 ±0.032,P＜0.05),OE4和OE6组阴道平滑肌细胞Rock2表达显著高于C4和C6(0.377 ±0.035 vs 0.294 ±0.033；0.388 ±0.038 vs 0.300 ±0.034,P＜0.05).结论 低雌激素水平上调RhoA/Rho激酶在大鼠阴道平滑肌中的表达,E2替代治疗可抑制RhoA/Rho激酶在去势大鼠阴道平滑肌中的高表达,雌激素可能通过调控RhoA/Rho激酶表达影响阴道平滑肌功能.

Effect of oestrogen on the expression of Rho-kinase in the vaginal smooth muscle cells of rats

Objective To determine the expression of RhoA/Rho-kinase in the vaginal smooth muscle cells of castrated rats and the effect of estrogen on the expression of RhoA/Rho-kinase. Methods 30 female SD rats of 10 weeks were randomly divided into groups C4(4-week control group),C6(6-week control group),OE4(4-week bilateral ovariectomy),OE6(6-week bilateralovariectomy),E4(4-week bilateral ovariectomy and oestrogen replacement) and E6(6-week bilateral ovariectomy and oestrogen replacement). 4 and 6 weeks after surgery, the serum estradiol level was determined and the expressions of Rock1 and Rock2 in the vaginal smooth muscle were detected by western blot.　Results Serum estradiol was significantly lower in group OE4 and OE6 than in groups C4 and C6( P < 0. 01) .RhoA/Rho-kinase was expressed in the vaginal smooth muscle cells of the rats. The protein expression of Rock1 was significantly more in groups OE4 and OE6 than in groups C4 and C6(0.261±0.039 vs 0.207±0.032；0.270±0.037 vs 0.208±0.032，P＜0.05), and so were Rock2 in groups OE4 and OE6 in comparison with groups C6 and E6(0.377±0.035 vs 0.294±0.033；0.388±0.038 vs 0.300±0.034，P＜0.05). Conclusion The replacement of oestrogen may inhibits the expression of RhoA/Rho-kinase in the vaginal smooth muscle cells of the rats,E2 may regulate the function of vaginal smooth muscle through regulate the expression of RhoA/Rho-kinase.