前列腺癌组织中的端粒酶活性表达

目的 探讨前列腺癌组织中端粒酶活性表达与前列腺癌某些生物学行为的着急。方法 采用端粒扩增文件－酶（ＴＲＡＰ＿ＥＬＩＳＡ）测定前列腺癌组织中端粒酶活性。结果 １０例正常前腺组织无端粒酶活性。１５例前腺腺癌组织中１３你检出端粒酶活性，阳性率８６％。端粒酶活性与肿瘤分化程度相关。１５例良性前列腺增生组织中４例检出端粒酶活性，阳性率为２６％，其水平显著低于肿瘤组织。结论 端粒酶活性与前列腺癌的生物学行为有

Expression of Telomerase Activity in Prostate Cancer

Objective To investigate the relationship between telomerase activity and the biological behavior of prostate cancer.Methods The telomerase activity in prostate cancer tissues was detected by TRAP( telomeric repeat amplification protocol) ELISA which is based upon amplification of the initial telomerase product and detected by ELISA.Results None of the 10 samples from normal prostates expressed telomerase activity. The thirteen of fifteen cases with prostate cancer presented telomerase acitivity. The activity of telomerase was associated with the pathological differentiation of prostate cancer. The four of fifteen cases with benign prostatic hyperplasia (BPH) expressed telomerase activity, and the activity of telomerase was lower than prostate cancer. Conclusions The telomerase activity may be related to biological behavior of prostate cancer and can evaluate the malignant potential of prostate cancer. The presence of telomerase acitvity in some cases of BPH may indicated that there are a few of malignant cells, as yet clinically undetected, within the tissue of BPH.