神经干细胞脑室内移植治疗大鼠脑缺血的实验研究

目的 探讨大鼠胚胎神经干细胞移植治疗局灶性脑缺血再灌注损伤的可行性。方法 取胎龄8～10d大鼠神经干细胞体外扩增．采用免疫组织化学方法检测神经干细胞及其分化后代的特异性标志巢蛋白（nestin）、胶质纤维酸性蛋白（GFAP）和神经元特异性烯醇化酶（NSE）的表达。以Brdu标记神经干细胞，分别于缺血后24h和4周移植至局灶性脑缺血大鼠模型的脑室内和梗死中心，比较不同移植部位和不同移植时间神经干细胞的存活、增殖和迁移情况。结果 移植后可见移植细胞存活至少8周以上．移植部位不同不影响细胞存活。脑室内移植细胞向脑缺血区域迁移．且以缺血后24h移植组较缺血后4周移植组迁移趋向性更强。结论 大鼠胚胎神经干细胞移植至局灶性脑缺血大鼠脑室内和梗死中心均可长期存活并广泛迁移．其迁移趋化能力与缺血后移植时间有关。

Experimental study of intraventricular neural stem cell transplantation for rat focal cerebral ischemia

Objective To explore the feasibility of neural stem cells(NSCs) transplantation for focal brain ischemia-reperfusion injury in rats.Methods Rat NSCs of 8 to 10 day gestational age were adopted for amplification in vitro,and detected by immunohistochemistry for the expression of nestin as a specific mark of NSCs,glial fibrillary acidic protein and neuron-specific enolase,and then implanted into the ventricle or central site in the infarcted area of the rat models 24 hours and 4 weeks after the ischemia respectively.Brdu labeling method was used to mark the transplanted NSCs and their offsprings.The differences in survival,proliferation,and migration were compared between the NSCs at different sites and time points for implantation.Results The transplanted NSCs lived for at least 8 weeks,which showed no relation with the transplanted site.Intraventricular infarcted NSCs migrated to the ischemic region,and the migration tendency was more significant in the 24-hour group than in the 4-week group.Conclusion NSCs from rat embryonic can live for a long period and migrate widely after transplantation into the ventricle or central site in the infarcted area in rats,and the migration tendency is related to the transplanted time after ischemia.