KIAA0280的亚细胞定位及在缺血脑组织中的表达

目的观察大鼠急性局灶性脑缺血时缺血组织与非缺血组织中KIAA0280蛋白的表达及亚细胞定位。方法应用免疫组织化学法测定KIAA0280在大鼠急性脑缺血后在缺血与非缺血区表达的差异,利用激光共聚焦扫描显微镜检测KIAA0280的亚细胞定位。结果免疫组织化学结果证实了KIAA0280在大鼠急性局灶性脑缺血后表达的差异,激光共聚焦扫描显微镜证实KIAA0280在大鼠皮层细胞。胶质细胞胞浆和细胞膜上均出现表达。KIAA0280在脑缺血后明显上调表达。结论KIAA0280与蛋白质合成和信号传递有密切关系,为新的脑缺血相关蛋白质,对其功能深入研究将对诊断和治疗脑缺血缺氧疾病奠定基础。

The expression of KIAA0280 protein in ischemic brain and its subcellular localization

Objective To observe the expression of KIAA0280 protein and its subcellular localization in ischemic and normal tissue of focal ischemic brain in rats. Methods Immunohistochemical method was used to test the different expression of KIAA0280 protein in ischemic and normal area. Confocal laser scanning microscope was used to observe its subcellular localization. Results Compared with the control side, the expression of KIAA0280 in ischemia lateral was higher. Confocal laser scanning microscope analysis showed that the KIAA0280 located in cytoplasm and membrane both in neurons and astrocytes. Conclusion These data provide insight into the new protein that participates in the ischemia-related stress response both in neuron and astrocytes.