| 细胞外基质支架对大鼠嗅鞘细胞神经营养素表达的影响 |
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| 引用本文： | 蒋锋,胡萍萍,沈丽萍,吴晓红,杨平. 细胞外基质支架对大鼠嗅鞘细胞神经营养素表达的影响[J]. 蚌埠医学院学报, 2010, 35(11): 1087-1090. DOI: 10.3969/j.issn.1000-2200.2010.11.003 |
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| 作者姓名： | 蒋锋 胡萍萍 沈丽萍 吴晓红 杨平 |
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| 作者单位： | 江苏省镇江市第二人民医院医教科, 212001 |
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| 摘 要： | 目的:构建一种复合型细胞外基质支架,并探讨其是否影响大鼠嗅鞘细胞(olfactory ensheathing cells,OEC)合成神经营养素。方法:将纤维蛋白原、层黏连蛋白和纤黏连蛋白按一定比例混合,在新鲜大鼠血浆促凝作用下,构建细胞外基质支架,在支架表面种植大鼠OEC,观察其生长增殖情况和对神经营养因子表达能力的影响。结果:OEC在复合支架上贴壁良好,增殖旺盛,伸出较长的突起。生长于复合支架上的OEC较玻璃片上的细胞合成更多的神经营养素。结论:细胞外基质支架能促进OEC增殖,同时促进OEC神经营养素分泌。
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| 关 键 词： | 嗅鞘细胞 纤维蛋白原 层黏连蛋白 纤黏连蛋白 神经营养素 大鼠 |
| 收稿时间： | 2009-06-22 |
The influence of extra cellular matrix scaffold on the expression of neurotrophins in rat olfactory ensheathing cells |
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| JIANG Feng,HU Ping-ping,SHEN Li-ping,WU Xiao-hong,YANG Ping. The influence of extra cellular matrix scaffold on the expression of neurotrophins in rat olfactory ensheathing cells[J]. Journal of Bengbu Medical College, 2010, 35(11): 1087-1090. DOI: 10.3969/j.issn.1000-2200.2010.11.003 |
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| Authors: | JIANG Feng HU Ping-ping SHEN Li-ping WU Xiao-hong YANG Ping |
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| Affiliation: | Department of Medical Affairs, Zhenjiang Second People′s Hospital, Zhenjiang Jiangsu 212001, China |
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| Abstract: | Objective:To construct a kind of extracellular matrix scaffold and investigate whether it can influence the expression of neurotrophins in rat olfactory ensheathing cells.Methods:Mixed fibrinogen,laminin and fibronectin proportionally,then added fresh rat plasma to construct scaffold.Plated the rat olfactory ensheathing cells(OEC) on scaffold and then observed the influence of scaffold on proliferation and neurotrophin expression of OEC.Results:Rat OEC adhered to scaffold easily,proliferated quickly and ext... |
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| Keywords: | olfactory ensheathing cells fibrinogen laminin fibronectin neurotrophins rat |
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