嗜酸性粒细胞在激素抵抗型哮喘中的作用

目的了解嗜酸性粒细胞在激素抵抗型哮喘(SRA)的发病机理和诊断中的意义。方法采集SRA患者和非激素抵抗型哮喘(NSRA)患者的外周血，Percoll密度梯度法分离嗜酸性粒细胞，分别应用DPH染色荧光分光光度计测量嗜酸性粒细膜粘性和罗丹明123染色流式细胞仪测定其线粒体膜电位。结果SRA患者低密度嗜酸性粒细胞(HE)比例增加，嗜酸性粒细胞的膜粘滞度增加，线粒体膜电位的相对荧光强度下降。结论功能活跃的HE增多可能是SRA发生的机理之-，嗜酸性粒细胞膜流动性及线体膜电位的改变，有助于SRA和其他哮喘的鉴别。

The role of eosinophil fluidity of membrane and mitochondrial membrane potetial in pathogenesis of steroid resistant asthma

Objective To investigate whether eosinophil plays a role in the pathogenesis of steroie resistant astma . Method Bloodspecimens from SRA and NSRA patients were collected, eosinophils were separated by Percoll' s liquid, The fluidity of DPH-stained eosinophilmembrane was measured by spectrofluorometer eosinophi; ' s mitochodrial membrane potential indicated by Rodamme 123was determined byflow cytometer. Result The ratio of hypodense eosinophil in SRA patients was higher than those in NSRA patients [ (17.5 ± 3.70)]%/(11.5 ± 3.03)% , P = 0.0016. ], The fluorescence laberled mitochondrial membrane potentials in SRA were decreased in contrast to those inNSRA[ (39.35 ± 12.43 / (57.35 ± 15.22), P = 0.021 ]. Conclusion Our data suggest that active hypodense eosinophil might contribute tothe pathogenesis of SPA, The esosinophil fluidity of membrane and mitochondrial membrane potential maybe used as.