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| 全反式维甲酸和1,25-二羟维生素D3联合应用对大肠癌细胞的分化诱导作用 | |
| 引用本文： | 郭俊明,何小洪,廖异平,罗超权. 全反式维甲酸和1,25-二羟维生素D3联合应用对大肠癌细胞的分化诱导作用[J]. 癌症, 1999, 18(6): 649-651 |
| 作者姓名： | 郭俊明 何小洪 廖异平 罗超权 |
| 作者单位： | 1. 中山医科大学生物化学教研室,广州,510089 2. 中山医科大学附属一院心内科实验室 3. 中山医科大学组胚学教研室 |
| 摘 要： | 目的：观察联合使用全反式维甲酸（all-trans-retinoic acid,ＡＴＲＡ）和１，２５－二羟维生素Ｄ３对大肠癌细胞株ＬoＶo的影响。方法：以ＭＴＴ法测定细胞生长，碱性磷酸酶（alkaline phosphatases,ＡＬＰ）比活性检测细胞分化。结果：ＡＴＡＲ和１，２５－二羟维生素Ｄ３对ＬoＶo生长的抑制作用在一定浓度范围内呈时间深度依赖关系，联合用药时抑制作用增强。经药物作用细胞后肠型ＡＬＰ逐渐升高。结论：联合使用ＡＴＲＡ逐渐升高 |
| 关 键 词： | 全反式维甲酸 1,25-二羟维生素D3 LoVo细胞 诱导分化 |
| 文章编号： | ：1000-467X(1999)06－0649－03 |
Differential effects of combinative using all-trans-retinoic acid and1,25-dihydroxy vitamin D3 on large intestinal cancer cells |
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| GUO Jun ming,HE Xiao hong,LIAO Yi ping,et al.. Differential effects of combinative using all-trans-retinoic acid and1,25-dihydroxy vitamin D3 on large intestinal cancer cells[J]. Chinese journal of cancer, 1999, 18(6): 649-651 | |
| Authors: | GUO Jun ming HE Xiao hong LIAO Yi ping et al. |
| Affiliation: | GUO Jun ming,HE Xiao hong,LIAO Yi ping,et al. Department of Biochemistry,Sun Yat sen University of Medical Sciences,Gunagzhou 510089,P.R.China |
| Abstract: | Objective:To observe the differentiation effects of combinative use of all trans retinoic acid (ATRA) and 1,25 dihydroxy vitamin D 3 on large intestinal cancer cells. Methods:Growth inhibition was studied using MTT assay. Differentiation was assessed by alkaline phosphatases (ALP) relative activities. Results:The inhibitions of ATRA and 1,25 dihydroxy vitamin D 3 on LoVo appeared in a dose and time dependent manners. The inhibition was increased as combinative use of both of them. The intestinal type of ALP was gradually increased by drug treatment. Conclusion:The differentiation effects of combinative use of ATRA and 1,25 dihydroxy vitamin D 3 on LoVo cells was better than that of use of any one inducer only. |
| Keywords: | All trans retinoic acid 1 25 dihydroxy vitamin D 3 LoVo cells Differentiation |
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