Bcl-2过度表达对大鼠成骨细胞Bax、Caspase-3表达的影响

目的：探讨凋亡相关基因Bcl-2过表达对大鼠成骨细胞Bax蛋白以及活性Caspase-3表达的影响。方法：将含有Bcl-2cDNA的逆转录病毒表达载体pDOR-SB质粒转染大鼠成骨细胞使之过表达，用免疫组化ABC法检测Bax、Caspase-3活性蛋白表达变化。结果：转染筛选后的阳性克隆细胞表现为Bcl-2表达显著增强。同时Bax蛋白表达下降接近显著性差异，活性Caspase-3表达无明显变化。结论：Bcl-2过度表达对活性Caspase-3表达无影响，但对Bax蛋白表达起下调作用，提示Bcl-2家族成员之间可以相互影响。

Effect of Overexpression of Bcl-2 on Bax and Caspase-3 Protein Expression in Rat Osteoblasts

Objective:To explore the effect of apoptosis-associated gene Bcl-2 overexpression on the expression of Bax and Caspase-3 protein in rat osteoblasts.Methods:The plasmid of retrovirus-expression vector pDOR-SB,which contains Bcl-2c DNA,was transfected into primary cultured calvaria osteoblasts of newborn rat and made it overexpressed.The expressions of Bcl-2,bax and Caspase-3 proteins were detected by immunohistochemical ABC method.Results:Bcl-2 protein expression was raised dramatically.Meanwhile,the expression of Bax was decreased and there was no difference in expression of Caspase-3 after transfection.Conclusion:Overexpression of Bcl-2 has no effect on the expression of Caspase-3,however,could reduce the expression of Bax,which indicated the members of Bcl-2 can influence each other by some way unknow to us now.