脐血输注后再生障碍性贫血、白血病患者造血祖细胞体外培养及血浆造血刺激活性变化

为探讨脐血输注的疗效机理，评价脐血输注的实用价值，我们用新鲜脐血静脉输注（ＵＣＢＴ），治疗慢性再生障碍性贫血（ＣＡＡ）１０例和急性白血病（ＡＬ）８例，观察ＵＣＢＴ前后患者粒单细胞系集落形成单位（ＣＦＵ－ＧＭ）、红系爆式集落形成单位（ＢＦＵ－Ｅ）、集落刺激活性（ＣＳＡ）、爆式集落刺激活性（ＢＰＡ）及外周血象变化。结果提示：ＵＣＢＴ后①可迅速升高ＣＦＵ－ＧＭ和ＢＦＵ－Ｅ；②可改善外周血象，尤其是ＷＢＣ、及ＡＮＣ和Ｈｂ；③可使病理性增高的ＣＳＡ、ＢＰＡ降至正常。结果提示可能系脐血中的祖细胞和造血因子刺激病人骨髓，并使造血功能得到改善。在某些病理情况下ＣＳＡ和ＢＰＡ的增高是机体的一种生物代偿反应，ＵＣＢＴ使病理增高的ＣＳＡ和ＢＰＡ显著下降并趋于正常可能是通过反馈调节作用而使细胞因子水平下降。

THE CHANGES OF HEMOPOIETIC PROGENITOR CELL IN VITRO CULTURE AND HEMOPOIETIC STIMULATION ACTIVITY IN PLASMA AFTER UMBILICAL CORD BLOOD TRANSFUSION IN PATIENTS WITH APLASTIC ANEMIA AND LEUKEMIA

Abstract:In order to better understand the effective mechanism of UCBT and estimate its practicalvalue, eighteen patients, ten with CAA and eight with AL, were treated by UCBT. The patients, BM CFU-GM, peripheral blood BFU-E, CSA and BPA in plasma were measured before and after treatment. The results showed as following:after treatment ①CFU-GM inAL group and BFU-E in CAA group increased markedly ②WBC, ANC ameliorated significantly in both groups and Hb in CAA group ③Pathological increase of CSA and BPA levelsin two groups reduced and trended to normal value.All of these results may be indicated that progenitors and hematopoietic factors in UCBstimulated patients'BM, and made the condition get well. High level of CSA and BPA can beseen in sone hematologic diseases, it is considered a reaction of biocompensation. AfterUCBT, enhanced colony formation, but declined the level of CSA and BPA, probably it isthrough the feedback regulation of the body.