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| 热应激对大鼠睾丸Bcl-2和Bax表达的影响 | |
| 引用本文： | 谢远杰,石金凤,赵国军,李美香,龙治峰,贺丽萍. 热应激对大鼠睾丸Bcl-2和Bax表达的影响[J]. 南华大学学报(医学版), 2010, 38(2): 166-169 |
| 作者姓名： | 谢远杰 石金凤 赵国军 李美香 龙治峰 贺丽萍 |
| 作者单位： | 1. 南华大学,组织学与胚胎学教研室,湖南,衡阳,421001 2. 南华大学,显微形态中心 3. 湖南师范大学,医学院组织学与胚胎学教研室 |
| 基金项目： | 湖南省科技厅基金资助(03SSY3106) |
| 摘 要： | 目的研究热应激对大鼠睾丸B细胞淋巴瘤/白血病-2(B cell lymphoma/leukemia-2,Bcl-2)和Bcl-2相关X(Bcl-2 associated X,Bax)表达的影响。方法42天龄24只雄性SD大鼠建立单侧隐睾作为热应激处理的模型,随机选取16只大鼠建立左侧隐睾作为热应激处理的模型,其余8只将左侧睾丸还纳入阴囊后关腹作为假手术组。术后第3天和第7天分别采用RT-PCR和免疫组织化学SABC方法检测热应激处理后睾丸Bcl-2、Baxm RNA和蛋白表达的变化。结果与假手术组睾丸相比,热应激后第7天睾丸生精上皮和睾丸间质细胞内Bax mRNA和蛋白表达增高,而Bcl-2 mRNA蛋白在热应激后第3天表达明显降低,差异有显著性(P<0.05)。结论热应激能导致睾丸内Bax表达增高,Bcl-2表达降低,从而促进生精细胞凋亡增加。 |
| 关 键 词： | 热应激 睾丸 Bcl-2 Bax RT-PCR |
Effect of Heat Stress on the Expression of Bax and Bcl-2 in the Rat Testis |
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| XIE Yuan-jie,SHI Jin-feng,ZHAO Guo-jun,et al. Effect of Heat Stress on the Expression of Bax and Bcl-2 in the Rat Testis[J]. Journal of Nanhua University(Medical Edition), 2010, 38(2): 166-169 | |
| Authors: | XIE Yuan-jie SHI Jin-feng ZHAO Guo-jun et al |
| Affiliation: | Department of Histology and Embryology;University of South China;Hengyang;Hunan 421001;China |
| Abstract: | Objective To investigate the effect of heat stress on the expression of B cell lymphoma /leukemia-2(Bcl-2) and Bcl-2 associated X (Bax) in the rat testis. Methods Unilateral cryptorchidism was surgically induced in the 42-day old rats as the model of heat stress.The expression of Bax and Bcl-2 was detected respectively by RT-PCR and immunohistochemical SABC assays on the 3rd and 7th day after the operation. Results Compared to the testis of the sham operation group the expression of Bax mRNA and protein in ... |
| Keywords: | heat stress testis Bcl-2 Bax RT-PCR |
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