| 小剂量左旋多巴上调pCREB及CD39蛋白表达发挥抗氧化应激损伤作用的研究 |
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| 引用本文： | 钟时颖,谈艳,陆悠,赵延欣,白雪,李强. 小剂量左旋多巴上调pCREB及CD39蛋白表达发挥抗氧化应激损伤作用的研究[J]. 中国临床神经科学, 2014, 0(1): 38-42 |
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| 作者姓名： | 钟时颖 谈艳 陆悠 赵延欣 白雪 李强 |
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| 作者单位： | 上海市同济大学附属第十人民医院神经内科,200072 |
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| 基金项目： | 上海市科委产学研医结合项目子课题：多巴丝肼胶囊对帕金森病神经元保护作用临床研究（编号：11DZ1920903） |
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| 摘 要： | 目的明确左旋多巴对PC12细胞生长及应激状态下存活的影响,探讨其抗氧化应激损伤的机制。方法不同浓度左旋多巴处理PC12细胞,用MTT法检测PC12细胞增长率及加入过氧化氢后细胞存活率;免疫荧光、Western blot方法测定磷酸化环单磷酸腺苷反应元件结合蛋白(pCREB)及CD39蛋白表达。结果低浓度左旋多巴(20μmol·L-1)促进PC12细胞生长,且可抗氧化应激损伤,而蛋白激酶抑制剂减弱此保护作用。免疫荧光及Western blot结果显示CD39及pCREB表达升高。结论低浓度左旋多巴可通过上调CD39及pCREB表达发挥抗氧化应激神经保护作用。
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| 关 键 词： | 左旋多巴 PC12细胞 氧化应激 神经保护 CD39 磷酸化环单磷酸腺苷反应元件结合蛋白 |
Small Doses of Levodopa Protect Nerve Cells from Oxidative Stress by Up-regulating pCREB and CD39 |
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| ZHONG Shi-ying. TAN Yan,LU You,ZHAO Yan-xin,BAI Xue,LI Qiang. Small Doses of Levodopa Protect Nerve Cells from Oxidative Stress by Up-regulating pCREB and CD39[J]. Chinese Journal of Clinical Neurosciences, 2014, 0(1): 38-42 |
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| Authors: | ZHONG Shi-ying. TAN Yan LU You ZHAO Yan-xin BAI Xue LI Qiang |
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| Affiliation: | ( Department of Neurology, Shanghai Tenth People Hospital, Shanghai Tongji University, Shanghai 200072, China) |
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| Abstract: | Aim To figure out the influence of small doses of L-dopa on PC12 cell growth and neuronal cell death under oxidative stress.Methods The Cells were cultured with different concentrations ofL-dopa.MTT assay was used to measure cell growth rate and survival rate after the treatment of H2O2.The expression of phosphorylated cAMP response element-binding protei（pCREB） and CD39 were determined by immunofluorescence test and Western blot.Results Low concentration of L-dopa can promote PC12 cells growth and has protection against oxidative stress.The protein kinase A （PKA） inhibitor can eliminate this effect.The immunofluorescence and Western blot showed that expression of CD39 and pCREB increased.Conclusion Low concentration of L-dopa can protect neural cells by up-regulating the expression of CD39 and pCREB. |
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| Keywords: | L-dopa PC12 cell oxidative stress neuroprotection CD39 phosphorylated cAMP response element-binding protein |
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