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| 诱导K562细胞分化对EDAG基因表达的影响 | |
| 引用本文： | 方芳,陈忠航,张宸豪,许望翔,马爱新. 诱导K562细胞分化对EDAG基因表达的影响[J]. 吉林医药学院学报, 2005, 26(4): 195-197 |
| 作者姓名： | 方芳 陈忠航 张宸豪 许望翔 马爱新 |
| 作者单位： | 1. 吉林医药学院病原教研室,吉林,吉林,132013 2. 吉林医药学院国有资产处,吉林,吉林,132013 3. 军事医学科学院放射医学研究所,北京,100805 |
| 摘 要： | 目的研究EDAG在红系肿瘤分化中的表达及意义。方法用PCR的方法从人的血液基因组中分离出EDAG基因的调控区2200bp片段,将其克隆到pGL3-basic的含有荧光素酶报告基因的质粒中,转染K562细胞,用Hemin和PMA诱导细胞分化;通过不同时间检测荧光素酶报道基因的方法,观察用两种不同方法处理细胞后对EDAG基因表达的影响。结果用Hemin和PMA分别诱导K56272h和48h后,EDAG基因表达下调。结论用Hemin和PMA诱导K562细胞分化后,EDAG基因表达下调可能与细胞分化有关。 |
| 关 键 词： | 基因表达 诱导分化 |
| 文章编号： | 1673-2995(2005)04-0195-03 |
| 修稿时间： | 2005-10-13 |
K562 cells affect on EDAG genic expression by inductor |
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| FANG Fang,CHENG Zhong-hang,ZHANG Zhen-hao,XU Wang-xiang,MA Ai-xin. K562 cells affect on EDAG genic expression by inductor[J]. Journal of Jilin Medical College, 2005, 26(4): 195-197 | |
| Authors: | FANG Fang CHENG Zhong-hang ZHANG Zhen-hao XU Wang-xiang MA Ai-xin |
| Abstract: | Objective To study EDAG gene expression in differentiation of k562 cells. Methods 2 200 bp fragment of the EDAG promoter region was isolated from human blood genome. PCR products were subcloned into the luciferase reporter vector pGL3-basic and transfect with K562 cells. K562 cells were induced by PMA and Hemin, the expression of EDAG gene in the resulted cells was determinded by luciferase assay. Results The expression of EDAG gene in K562 cells was downregulated by Hemin and PMA-inducing for 72 and 48 hours respectively. Conclusion Expressional downregulation of EDAG gene is related to be cell differentiation by PMA and Hemin inducing. |
| Keywords: | EDAG |
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