| 壳聚糖微球固定化重组TEM-116型ESBL的研究 |
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| 引用本文： | 徐荣,韩冬青,王震,楼永良,陈秀枢.壳聚糖微球固定化重组TEM-116型ESBL的研究[J].中国抗生素杂志,2011,36(1). |
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| 作者姓名： | 徐荣 韩冬青 王震 楼永良 陈秀枢 |
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| 作者单位： | 温州医学院微生态学研究所,温州,325000 |
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| 基金项目： | 国家高科技发展"863"计划 |
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| 摘 要： | 目的 利用固定化酶技术,使重组TEM-116型超广谱β-内酰胺酶(ESBL)在清除环境中残留抗生素的应用中可重复使用,并改善其稳定性.方法 以戊二醛为交联剂将重组TEM-116型ESBL固定于壳聚糖微球载体,并采用单因素轮换法和响应曲面法对固定化条件进行优化.结果 4℃条件下,当戊二醛浓度0.54%、交联pH5.4、给酶量0.58IU/g壳聚糖微球、固定化pH6.2时,同定化酶的酶活和回收率分别可达到0.49IU/g和84%(0.49IU/0.58IU).与游离酶相比,固定化酶的酸碱稳定性和可重复利用性得到显著提高,在pH5.8～7.4范围内酶活性保持在73%～100%的水平,经7次重复性使用,每次1h,酶活力保留高达80%.结论 通过固定化酶技术,以重组TEM-116型ESBL为代表的耐药酶能够开发为具有广泛应用前景的环保制剂,以消除外环境中残留的抗生素.
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| 关 键 词： | 壳聚糖 固定化酶 超广谱β-内酰胺酶 |
Study on immobilization of recombinant TEM-116 ESBL with chitosan microspheres |
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| Xu Rong,Han Dong-qing,Wang Zhen,Lou Yong-liang,Chen Xiu-shu.Study on immobilization of recombinant TEM-116 ESBL with chitosan microspheres[J].Chinese Journal of Antibiotics,2011,36(1). |
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| Authors: | Xu Rong Han Dong-qing Wang Zhen Lou Yong-liang Chen Xiu-shu |
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| Institution: | Xu Rong,Han Dong-qing,Wang Zhen,Lou Yong-liang and Chen Xiu-shu(Institute of Microecology,Wenzhou Medical College,Wenzhou 325000) |
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| Abstract: | Objective To improve the stability of recombinant TEM-116 ESBL and make it reusable in clearing the environmental residual antibiotics through immobilization.Methods Recombinant TEM-116 ESBL was immobilized on chitosan microspheres by glutaraldehyde-crosslinking method,then the immobilizing conditions were optimized by means of one-factor-at-a-time experimentation and response surface methodology.Results The activity and the recovery rate of the immobilized TEM-116 ESBL were up to 0.49IU/g and 84%(0.49IU/0.... |
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| Keywords: | Chitosan Immobilized enzyme ESBL |
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