| 青霉素高产菌株高通量检测和筛选方法的研究 |
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| 引用本文： | 王丽丽,张春晓,刘晓红,陈晓波.青霉素高产菌株高通量检测和筛选方法的研究[J].中国抗生素杂志,2011,36(3). |
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| 作者姓名： | 王丽丽 张春晓 刘晓红 陈晓波 |
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| 作者单位： | 河北科技大学生物科学与工程学院,石家庄,050018 |
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| 基金项目： | 国家"十一五"科技支撑计划课題 |
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| 摘 要： | 目的:建立高通量筛选青霉素高产菌株的方法.方法:采用青霉素酶水解青霉素形成青霉噻唑酸,产物与碘液反应,以淀粉为指示剂,青霉素含量与碘液用量呈现良好的线性关系.结果:在室温条件下,不超过10min,可并行检测多个发酵液样品的青霉素含量,该方法简便、迅速、微量并且不受发酵液中其他杂质成分的干扰,也可在10min内对超过200个发酵液样品中的青霉素含量进行相对评估,淘汰90%以上的低效价菌株.结论:提供了高通量筛选青霉素高产菌株的方法.
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| 关 键 词： | 青霉素 青霉素酶 高通量检测 高通量筛选 |
A high-throughput penicillin titer detecting method and its application in high-throughput screening of penicillin-producing strains |
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| Wang Li-li,Zhang Chun-xiao,Liu Xiao-hong,Chen Xiao-bo.A high-throughput penicillin titer detecting method and its application in high-throughput screening of penicillin-producing strains[J].Chinese Journal of Antibiotics,2011,36(3). |
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| Authors: | Wang Li-li Zhang Chun-xiao Liu Xiao-hong Chen Xiao-bo |
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| Institution: | Wang Li-li,Zhang Chun-xiao,Liu Xiao-hong and Chen Xiao-bo(College of Biology Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018) |
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| Abstract: | Objective To estabolish a rapid penicillin titer detecting method to facilitate the high-throughput screening of penicillin-producing strains.Methods Penicillinase was employed to hydrolysis penicillin to penicillanic acid,which would react with iodine.Results The penicillin titer can be quantified within 10min at room temperature regardless of other by-products in the fermentation broth.In addition,and more than 200 samples can be simultaneously assessed by one operator within 10min.Conclusion This novel m... |
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| Keywords: | Penicillin Penicillinase High-throughput detection High-throughput screening |
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