| 梧宁霉素C组分高产突变株的获得和产物初步鉴定 |
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| 引用本文： | 黄婷娇,倪现朴,夏焕章. 梧宁霉素C组分高产突变株的获得和产物初步鉴定[J]. 中国抗生素杂志, 2011, 36(2) |
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| 作者姓名： | 黄婷娇 倪现朴 夏焕章 |
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| 作者单位： | 沈阳药科大学生命科学与生物制药学院,沈阳,110016 |
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| 摘 要： | 目的 选育抗真菌抗生素梧宁霉素高产菌株.方法 本文通过紫外线诱变的方式对不吸水链霉菌梧州亚种04(Streptomyces ahygroscopicus subsp.Wuzhouensis 04)进行菌种选育,并使用白念珠菌作为生物活性鉴定菌筛选高产突变株.结果 经筛选得到一株具有很高抑菌活性的突变株,在发酵液表观粘度、菌丝形态、组分分布等方面与出发菌株有明显差异.结论 经高效液相色谱及飞行时间质谱对产物的分析,证实该突变株为原出发菌株中含量较低的茴香霉素的高产菌株.
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| 关 键 词： | 不吸水链霉菌 抗真菌抗生素 紫外诱变 茴香霉素 |
The screening of high yield strains of Wuningmeisu C and the identification of its products |
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| Huang Ting-jiao,Ni Xian-pu,Xia Huan-zhang. The screening of high yield strains of Wuningmeisu C and the identification of its products[J]. Chinese Journal of Antibiotics, 2011, 36(2) |
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| Authors: | Huang Ting-jiao Ni Xian-pu Xia Huan-zhang |
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| Affiliation: | Huang Ting-jiao,Ni Xian-pu and Xia Huan-zhang(School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016) |
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| Abstract: | Objective To breed antifungal antibiotic wuningmeisu C high yield strain.Methods Streptomyces ahygroscopicus subsp.wuzhouensis 04 was mutated by UV mutagenesis.The bioactivity of high yield mutants was tested by the biological assay using Candida Albicans.Results A high yield strain was obtained by screening which was different from the original strain on the facet of apparent viscosity of the fermentation broth,the hyphal and the component distribution etc.Conclusion The analysis by high performance liquid... |
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| Keywords: | Streptomyces ahygroscopicus Antifungal antibiotic UV mutagenesis Anisomycin |
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