饱和游离脂肪酸通过其代谢产物酰基辅酶A导致卵巢颗粒细胞凋亡

探讨脂肪酸对人卵巢颗粒细胞生长的影响。不同剂量的游离脂肪酸处理颗粒细胞1－3天，用台盼蓝排除法对活细胞进行计数。用染色体DNA断裂和细胞形态学改变确定细胞凋亡。检测脂肪酸代谢产物酰基辅酶A是否直接引起细胞凋亡。测定与细胞凋亡相关的Bcl－2和Bax蛋白质表达水平。结果表明，饱和脂肪酸通过其代谢产物酰基辅酶A呈剂量依赖性的引起颗粒细胞凋亡，并导致Bcl-2水平下降和Bax水平升高。说明饱和脂肪酸对卵巢颗粒细胞有直接致凋亡作用。提示，女性肥胖和胰岛素抵抗患者出现的性功能障碍可能与饱和脂肪酸水平增高有关。

APOPTOSIS OF HUMAN OVARY GRANULOSA CELLS INDUCED BY THE METABOLITES ACYL-COA OF SATURATED FREE FATTY ACIDS

To investigate the apoptotic effect of free fatty acids (FFAs) on human ovary granulosa cells, the granulosa cells were treated with FFAs in various concentrations for three days, and then the cell viability was determined by trypan blue exclusion method. DNA fragmentation was examined by DNA ladder formation and Annexin V GFP/PI staining of the cells. We also determined if the apoptotic effect of saturated FFAs was mediated by their acly CoA metabolites and measured the expression of apoptosis related genes, Bcl 2 and Bax by Western blot. The results indicated that saturated FFAs induced apoptosis of granulosa cell by their acly CoA metabolites in a dose dependent manner, and led to the down regulation of Bcl 2 and the up regulation of Bax. It is concluded that saturated FFAs can induce apoptosis of human ovary granulosa cells, suggesting that the sexual disorder of obese and insulin resistant women is related to it.