| 大鼠脑组织缺血再灌注损伤及丹参干预作用实验研究 |
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| 引用本文： | 石萍,陈相潘,郑永.大鼠脑组织缺血再灌注损伤及丹参干预作用实验研究[J].西南国防医药,2008,18(3):328-331. |
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| 作者姓名： | 石萍 陈相潘 郑永 |
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| 作者单位： | 1. 重庆市中山医院药剂科,重庆,400013
2. 重庆医科大学附属第二医院临床药理基地,重庆,400010 |
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| 摘 要： | 目的：观察大鼠脑组织缺血再灌注后脑组织损伤变化及丹参的作用。方法：将42只SD大鼠随机分为正常组6只、缺血再灌注组（缺血组）18只和丹参治疗组（丹参组）18只，血管阻断法制作大鼠左侧脑缺血再灌注模型。丹参组于再灌注开始时腹腔注射丹参注射液（5ml／k），而缺血组注入等量生理盐水。观察时点为脑缺血再灌注后12h、24h和48h，观察大鼠脑组织病理变化，检测脑组织含水率及细胞凋亡情况（TUNEL法），检测脑组织丙二醛（malondialdehyde，MDA）、谷氨酸（glutamate，GLU）和7氨基丁酸（gamin-aminobutyric，GABA）含量变化。结果：与正常组比较，缺血组脑组织含水率、凋亡细胞数、MDA水平和GLU含量显著升高，GABA含量显著降低。与缺血组同时点比较，丹参组脑组织病理变化较轻，脑组织含水率、凋亡细胞数、MDA水平和GLU含量显著降低，GABA含量显著升高。结论：血管阻断法能造成显著的大鼠脑缺血再灌注损伤，丹参能有效降低其损伤效应。
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| 关 键 词： | 脑 再灌注损伤 凋亡 水肿 |
| 文章编号： | 1004-0188(2008)03-0328-03 |
| 修稿时间： | 2008年2月13日 |
Effects of salvia miltiorrhiza on ischemia reperfusion injury of brain in rats |
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| SHI Ping,CHEN Xiang-pan,ZHENG Yong.Effects of salvia miltiorrhiza on ischemia reperfusion injury of brain in rats[J].Medical Journal of National Defending forces in Southwest China,2008,18(3):328-331. |
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| Authors: | SHI Ping CHEN Xiang-pan ZHENG Yong |
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| Institution: | SHI Ping1,CHEN Xiang-pan1,ZHENG Yong2 1.Pharmaceutical Preparation Section of Zhongshan Hospital of Chongqing City,Chongqing,400013,China,2.Department of Pharmacy,Second Affiliated Hospital of Chongqing Medical University,400010,China. |
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| Abstract: | Objective:To observe the changes of brain tissue after ischemia reperfusion injury and to investigate the protective effects of salvia miltiorrhiza(SM).Methods:42 SD rats were divided into normal group(n=6),ischemia reperfusion injury group(control group,n=18) and SM group(n=18) at random.The model of ischemia reperfusion injury was made by occlusion of middle cerebral artery.SM injection was given to the rats in SM group through peritoneal injection at the dose of 5 ml/kg.The normal saline was given to the... |
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| Keywords: | brain ischemia reperfusion injury salvia miltiorrhiza treatment |
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