流式细胞术检测HLA-B27抗原的表达及意义--与微量淋巴毒实验的比较

目的对不同HLA-B27检测方法之间的差异及各自的优缺点进行评价并探讨其临床意义.方法分别采用微量淋巴毒法（MCLT）及流式细胞术（FCM）检测134例患者外周血标本的HLA-B27抗原,将二者结果进行分析比对;并对患者人群中的B27荧光强度与发病年龄及患病时间之间进行相关分析.结果两种检测方法检测脊柱关节病组和对照组HLA-B27阳性例数及阳性率相比,差异均无统计学意义,结果符合率为94.8%,仅有7例不符合,而对照组的符合率为100%;B27荧光强度与起病年龄之间存在较弱的正相关（r=0.345,P=0.026）,而与患病时间之间存在较弱的负相关（r=0.371,P=0.006）.结论两种检测方法均准确可靠,HLA-B27检测对AS的早期诊断与判断预后意义重大;B27表达强度与发病年龄及患病时间之间可能存在一定程度的关联.

Significance and detection of expression on HLA-B27 antigen by flow cytometry:comparison of microlymphocytotoxicity test

Objective To evaluate the differences,advantages and disadvantages of serology microlymphocytotoxicity test(MCLT) and flow cytometry(FCM) for detecting A-B27,and to investigate the clinical significance of detection on HLA-B27.Methods A total of 134 samples from patients were entered into the study,two methods(MCLT and FCM) were used to detect the expression of HLA-B27 antigen,and the results were analyzed;meanwhile the relationship between the intensity of B27 expression,occurrence age of patients and course of disease.Results The coincide rate of positive HLA-B27 of two methods was 94.8%,only 7 cases had different results,however the coincide rate of positive HLA-B27 of two methods was 100% in control.meanwhile the intense of B27 expression had a weak positive correlation with occurrence age of patients(r=0.345,P=0.026),and it had a weak negative correlation with course of disease(r=0.371,P=0.006).Conclusion MCLT and FCM prove to be accurate and reliable techniques,suitable for clinical application:Detection of HLA-B27 has a remarkable clinical significance on early diagnos is and prognosis of ankylosing spondylitis.There is the possible correlation between the intensity of B27 expression and occurrence age of patients,and course of disease.