Antioxidative function and biodistribution of [Gd@C82(OH)22]n nanoparticles in tumor-bearing mice |
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Authors: | Wang Jiangxue Chen Chunying Li Bai Yu Hongwei Zhao Yuliang Sun Jin Li Yufeng Xing Gengmei Yuan Hui Tang Jun Chen Zhen Meng Huan Gao Yuxi Ye Chang Chai Zhifang Zhu Chuanfeng Ma Baocheng Fang Xiaohong Wan Lijun |
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Affiliation: | Lab for Bio-Environmental Health Sciences of Nanoscale Materials and Key Lab of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, P.O. Box 918, Beijing 100049, PR China. |
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Abstract: | Oxidative stress is considered to be one of the important mechanisms involved in carcinogenesis. In our previous study, gadolinium endohedral metallofullerenol ([Gd@C82(OH)22]n nanoparticles) have shown high inhibitory activity on hepatoma cell (H22) growth in mice. To explore the antioxidative functions of nanoparticles, we investigated the biodistribution of [Gd@C82(OH)22]n nanoparticles, the changes of blood coagulation profiles, the metabolism of reactive oxygen species (ROS) in the tumor-bearing mice as well as the possible relationships between nanoparticles treatment and ROS production in this paper. The activities of hepatic superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) and catalase (CAT) as well as the levels of reduced glutathione (GSH), protein-bound thiols and malondialdehyde (MDA) were compared between the tumor-bearing mice and normal mice. Transplanted tumors were grown in mice by subcutaneous injection of murine hepatoma cells in the mice. The comparison of the above parameters between nanoparticles and cyclophosphamide (CTX) therapy were also investigated. [Gd@C82(OH)22]n administration can efficiently restore the damaged liver and kidney of the tumor-bearing mice. All the activities of enzymes and other parameters related to oxidative stress were reduced after [Gd@C82(OH)22]n treatment and tended closely to the normal levels. The results suggest that [Gd@C82(OH)22]n nanoparticle treatment could regulate ROS production in vivo. |
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Keywords: | CAT, catalase CDNB, 1-chlorinechloro-2,4-binitrobenzenedinitrobenzene CTX, cyclophosphamide DTNB, 5,5′-dithiobis 2-nitrobenzoic acid GSH, glutathione GSH-px, glutathione peroxidase GST, glutathione S-transferase 0" alt=" radical dot" src=" http://cdn.els-cdn.com/sd/entities/rad" class=" glyphImg" >OH, hydroxyl radical H2O2, hydrogen peroxide ICP-MS, inductively coupled plasma-mass spectrometry MDA, malondialdehyde NTP, 2-nitro-5-thiobenzoate anion 1O2, singlet oxygen O2 0" alt=" radical dot" src=" http://cdn.els-cdn.com/sd/entities/rad" class=" glyphImg" >−, superoxide anions PBS, phosphate buffer solution ROS, reactive oxygen species -SH, protein thiols SOD, superoxidase dismutase TBARS, thiobarbituric acid-reactive substance |
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