HPLC法测定强筋健骨散中羟基红花黄色素A的含量

目的建立强筋健骨散中羟基红花黄色素A的HPLC测定方法。方法色谱柱为MUCLEODUR C18 （4.6 mm × 250 mm,5μm），柱温35℃ ;流动相为乙腈-0. 1%磷酸溶液（11：89）,流速1. 0 ml·min ^- 1 ;检测波长403 nm。结果 羟基红花黄色素A保留时间约为19 min,与相邻峰的分离度大于1.5。以峰面积（Y）对进样浓度（X，μg？ml^-1）线性回 归，回归方程为Y=0. 033 91 X ＋3. 191，r =0.9998，线性范围 16.00 - 160.0 μg ·ml^-1加样回收率为 102.6%，RSD 为 3.2%。结论本方法操作简便，测定结果准确可靠，可用于强筋健骨散中羟基红花黄色素A的含量测定。

Determination of Hydroxysaffior Yellow A in Qiangjin Jiangu Powder by HPLC

Objective To establish an HPLC quantitative method for the determination of hydroxysafflor yellow A in Qiangjin Jiangu powder. Methods HPLC was used for the content determination of hydroxysafflor yellow A. The chromatography column was MUCLEODUR C18 （250 mm ×4. 6 mm,5 μm）and the column tempera-ture was 35 ℃. The mobile phase consisted of acetonitrile -0. 1% phosphoric acid（11＊ 89）. The flow rate was 1. 0 ml·min^-1 and monitored at 403 nm. Results The retention time of hydroxysafflor yellow A was about 19 min. The resolution rate was more than 1. 5. The regression equation for hydroxysafflor yellow A was Y =0. 033 91 X ＋ 3. 191,r = 0. 9998,and the linear range was 16. 00 - 160. 0 μg ·ml^-1. The average recovery of hydroxysafflor yellow A was 102. 6% ,while RSD was 3. 2% . Conclusion This method is simple,time-saving and accurate. It can be used for routine analysis of hydroxysafflor yellow A in Qiangjin Jiangu powder.