HPLC法测定麝香接骨胶囊中四种生物碱的含量

目的建立HPLC法测定麝香接骨胶囊中伪麻黄碱、麻黄碱、马钱子碱和士的宁的含量。方法色谱柱：SHI- MADZU VP-ODS C18柱（150 mm×4.6 mm，5μm）;流动相：乙腈（A）4. 01 mol·L^-1庚烷磺酸钠与 0. 02 mol·L^-1磷酸二氢钾等量混合溶液（B）（用10%磷酸调节pH值2.8）（19：81）;检测波长：206 nm;流速：1.0ml·min^-1;柱温：35℃。结果伪麻黄碱 在1.51 -48.4 ng、麻黄碱在16.09-515 ng、马钱子碱在12. 67 -405 ng、士的宁在16.29 -521.25 ng范围内线性关系良好;平 均回收率分别为伪麻黄碱96. 87% （RSD为1. 21% ）、麻黄碱99.12% （RSD为1.69% ）、马钱子碱101. 66% （RSD为2. 85% ）、 士的宁96.35% （RSD为2.74%）,线性范围分别为。结论此方法简便，准确，灵敏，可用于麝香接骨胶囊中麻黄和马钱子的 质量控制。

Determination of four Alkaloids in Shexiang Jiegu Capsules by HPLC

Objective To develop a method for the determination of ephedrine, pseudoephedrine, brucine and strychnine in Shexiang Jiegu capsules. Methods SHIMADZU VP-ODS C18 （150 mm × 4. 6 mm, 5 μm） was used. The mixed solution（pH was adjusted to 2. 8 with phosphate acid）composed of 0. 01 mol ·L^-1 sodium-heptane- sulfonate solution and an equal quantity of 0. 02 mol·L^-1 potassium dihydrgen phosphate solution was chosen as mobile phase B while acetonitrile was used as mobile phase A to elute in a gradient program of A-B（ 19： 81 ） . The detection wavelength was 206 nm and the flow rate was 1. 0 ml ？min ^- 1, with the column temperature at 35℃ . Re-sults Good linear relationships were obtained within the ranges of 1. 51 -48. 4 ng for pseudoephedrine,16. 09 -515 ng for ephedrine, 12. 67 -405 ng for brucine and 16. 29 -521. 25 ng for strychinine. The average recoveries（ n = 6） were 96. 87% （ RSD =2. 21% ） for pseudoephedrine,99. 12% （RSD =2. 69% ） for ephedrine, 101. 66% （ RSD =2. 85% ） for brucine,and 96. 35% （ RSD - 2. 74% ） for strychnine. Conclusion This method is simple, accurate and sensitive. It can be used for quality control of herba ephedrae and semen strychni in Shexiang Jiegu capsules.