甲磺酸伊马替尼对K562细胞bcr/abl、SHIP-2基因表达的影响及意义 |
| |
引用本文: | 刘小军,罗建民,杨林,武学文,温树鹏. 甲磺酸伊马替尼对K562细胞bcr/abl、SHIP-2基因表达的影响及意义[J]. 河北医药, 2010, 32(7): 771-773 |
| |
作者姓名: | 刘小军 罗建民 杨林 武学文 温树鹏 |
| |
作者单位: | 河北医科大学第二医院血液内科,石家庄市,050000;河北医科大学第二医院血液内科,石家庄市,050000;河北医科大学第二医院血液内科,石家庄市,050000;河北医科大学第二医院血液内科,石家庄市,050000;河北医科大学第二医院血液内科,石家庄市,050000 |
| |
基金项目: | 国家自然科学基金资助项目 |
| |
摘 要: | 目的研究甲磺酸伊马替尼对K562细胞bcr/abl、SHIP2的表达影响及意义。方法采用定量PCR方法检测甲磺酸伊马替尼干预K562细胞后bcr/abl、SHIP2基因表达变化。采用MTT及Western blot方法分别检测细胞增殖与AKT磷酸化水平的变化。结果随着甲磺酸伊马替尼浓度增加及作用时间的延长bcr/abl基因的表达下降(P〈0.01),SHIP2的表达增加(P〈0.01)。Akt磷酸化水平降低,细胞增殖能力降低。结论甲磺酸伊马替尼能下调bcr/abl基因的表达;SHIP2表达可能受bcr/abl基因的调节,并可能通过调节PI3K/AKT途径影响细胞的增殖。
|
关 键 词: | 白血病 髓系 慢性 BCR-ABL阳性 甲磺酸伊马替尼 基因表达 |
The influence and significance of Gleevic on the expression of bcr/abl and SHIP2 gene in K562 cell line |
| |
Affiliation: | LIU Xiaojun,LUO Jianmin,YANG Lin,et al.Department of Hematology,The Secongd Hospital of Hebei Medical University,Shijiazhuang 050000,China |
| |
Abstract: | Objective To investigate the influence and significance of Gleevic on the expression of bcr/abl and SHIP2 gene in K562 cell line.Methods The quantitative PCR was used to detect the expression of bcr/abl and SHIP2 gene in K562 cells after treated with Gleevic,and MTT and Western Blot were used respectively to detect the proliferation and phosphor-Akt levels of K562 cells after treated with Gleevic.Results With the increase of concentration of Gleevic and the prolangation of action time,the expression of bcr/abl gene was decreased in K562 cells,however,the SHIP2 gene expression was increased.The cell proliferation decreased and the levels of phosphor-Akt was decreased.Conclusion Gleevic can down-regulate the expression of bcr/abl gene.The expression of SHIP2 may be regulated by bcr/abl gene,which may affec cell proliferation by way of PI3K/AKT. |
| |
Keywords: | leukemia medullary system chronic BCR-ABL positive Gleevic the expression of gene |
本文献已被 维普 万方数据 等数据库收录! |
|