急性出血坏死性胰腺炎大鼠血清一氧化氮含量变化及硝普钠的治疗作用

为探讨急性出血坏死性胰腺炎（ＡＨＮＰ）大鼠血清一氧化氮（ＮＯ）的动态变化及硝普钠的治疗作用。将１２０只Ｗｉｓｔａｒ大鼠随机分为３组，每组各４０只。ＡＨＮＰ组动物模型采用经胰胆管逆行注射５％牛磺胆酸钠建立；治疗组为在ＡＨＮＰ模型制备后经腹腔注射硝普钠（０．０５ｍｇ／１００ｇ），３小时后再重复注射１次；对照组为大鼠开腹经胰胆管逆行注射生理盐水０．５ｍｌ后关腹。动态观察各组指标的变化。结果显示：ＡＨＮＰ组大鼠血清ＮＯ和超氧化物歧化酶（ＳＯＤ）含量明显低于治疗组（Ｐ＜０．０５）；血清淀粉酶和胰腺系数则明显高于治疗组（Ｐ分别＜０．０１和＜０．０５）；此外，ＡＨＮＰ组２４小时病死率明显高于治疗组，存活时间明显短于治疗组（Ｐ＜０．０１）；ＡＨＮＰ组胰腺的病理改变也明显重于治疗组。由此表明：ＮＯ在急性出血坏死性胰腺炎的发病过程中起了重要作用，硝普钠对大鼠ＡＨＮＰ有治疗作用

CHANGES OF SERUM NITRIC OXIDE LEVELS AND THERAPEUTIC EFFECTS OF SODIUM NITROPRUSSIDE IN ACUTE HEMORRHAGIC NECROTIZING PANCREATITIS IN RATS

To investigate changes of serum nitric oxide (NO) levels and therapeutic effects of sodium nitroprusside (SNP) in acute hemorrhagic necrotizing pancreatitis (AHNP) in rats, one hundred and twenty Wistar rats were divided into three groups randomly (forty in each group) animal models of AHNP group were produced by retrograde injection of 5% sodium taurocholate into pancreatobiliary duct.In the treatment group SNP (0.05mg/100g) was injected intraperitoneally after AHNP models established and then repeated three hours later. In the control group only retrograde injection of normal saline (0.5ml) into pancreatobiliary duct was performed. The results showed: compared with the treatment group, serum NO and SOD levels were significantly lower in AHNP group ( P <0.05); serum amylase levels and pancreatic indexes were markedly higher in AHNP ( P <0.01 and P <0.05 respectively);the mortality in 24 hours was significantly higher and survival time was significantly shorter in AHNP group ( P <0.01).Pathologic changes of pancreas were significantly severe in AHNP group. From the above results we can draw the conclusion that NO plays an important role in the pathogenesis of AHNP and SNP has therapeutic effects on AHNP in rats.