石菖蒲水煎剂对癫痫大鼠脑部神经发生及认知功能的影响

目的：探讨石菖蒲水煎剂对癫痫大鼠脑部神经发生的影响和对癫痫认知障碍的治疗作用。方法：SD大鼠30只，随机分为正常对照组、癫痫模型组和石菖蒲灌胃组，每组10只。癫痫模型组和石菖蒲灌胃组采用印防己碱（PTX）建立癫痫动物模型，采用5一溴脱氧尿苷嘧啶（-brolTlO-2-deoxyuridine，BrdU）腹腔注射标记内源性神经干细胞（NSC）。正常对照组、癫痫模型组予生理盐水灌胃，石菖蒲灌胃组予石菖蒲水煎剂10mg／（kg·d）灌胃，共30天。治疗前后进行鼠癫痫发作Racine评级、皮层脑电图检测、脑内BrdU阳性细胞免疫组化检测和Morris水迷宫检测。结果：Morris水迷宫检测显示，癫痫模型组逃避潜伏时间长于石菖蒲灌胃组和正常对照组（P〈0．05）；癫痫模型组目标象限内的游泳时间占整个游泳时间的百分率明显低于石菖蒲灌胃组和正常对照组（P〈0．05）。石菖蒲灌胃组大鼠Ⅲ～Ⅴ级癫痫发作频率、皮层脑电图频率、波幅降低、Brdu阳性细胞数升高，与癫痫模型组比较，差异有统计学意义（P〈0．05）。结论：大鼠发生癫痫后，启动自我保护机制，石菖蒲干预后明显增强了保护机制。

Effect of Water Decoction of Rhizoma Acori Tatarinowii on Epilepsy Rats' Brain and Cognitive Function LI

Objective：To explore the effect of the water decoction of rhizoma acori tatarinowii on brain neurogenesis and cognitive handicap of epilepsy in rats. Methods：Thirty SD rats were randomly divided into 3 groups,including control group,model group,and intervention group. Epilepsy model was established by picrotoxin in the model and intervention groups. Rats in the intervention group was given the water decoction of rhizoma acori tatarinowii of 10 mg/（kg＇d） for 30d. Saline was given in the same way as described in the intervention group to the control and model groups. Studies on SVZ and DG region of BrdU positive ceils in rat brain and immunohistochemical detec- tion and Morris water maze experiment were carried out. Results：Morris water maze results showed that escape la tency time of the model group was longer than that of the intervention group and the control group（P〈0.05）. In spatial probe test, the percentage of the swimming time of the model group was significantly lower than that of the intervention and control groups（P〈0.05）. Cortical EEG results revealed that the Rhizoma acori tatarinowii interven tion improved the cerebral cortex stimulation threshold（P〈0.05）. Brdu＋ cell number of the intervention group signif icantly increased as compared with that of the model group（P〈0.05）. Conclusion：Epilepsy rats start the self-pro- tection mechanism,and the rhizoma acori tatarinowii intervention can obviously enhance the protection mechanism.