甲基叔丁基醚对加油站职业人群血清淋巴细胞DNA的损伤

目的]探索汽油添加剂甲基叔丁基醚（methyltert—butylether，MTBE）对加油站职业暴露人群DNA的损伤。方法]选择华南地区8家加油站工作人员100名为调查对象（其中暴露组61人，对照组39人），进行健康检查和问卷调查；调查对象各抽取外周静脉血样5mL，分离血清和淋巴细胞，运用气相色谱一质谱（GC／MS）联用技术测定血清MTBE含量；运用彗星试验（cometassay），分析外周静脉血淋巴细胞DNA损伤情况；运用Pearson相关分析探索样本人群血清MTBE含量与淋巴细胞DNA损伤的相关性。结果]接受体检的加油站调查对象未发现身体健康状况不合格者，均符合汽油从业人员的职业卫生要求；暴露组血清MTBE含量平均值为（6．230±2．369）μg／L，高于对照组（5．164±2．139）μg/L]（P〈O．05）；暴露组0live尾矩平均值为（0．060±0．045）gm，也高于对照组I（0．039±0．038）μm]P〈0．05）；Pearson相关分析显示，暴露组血清MTBE含量与淋巴细胞DNA损伤程度之间呈正相关关系（r=0．859，P〈0．05）。结论]血清MTBE含量与淋巴细胞DNA损伤可能存在相关性。

Lymphocyte DNA Damage Induced by Methyl Tert-Butyl Ether in Gas Station Attendants

Objective 3 To probe the DNA damages induced by a gasoline additive, methyl tert-butyl ether （MTBE）, in occupational exposed population. Methods ] Physical examination and questionnaire survey were conducted in 100 workers selected from 8 gas stations in South China. A 5 mL peripheral venous blood sample was collected from each worker. Serum and lymphocytes were separated. The content of serum MTBE was detected with gas chromatography-mass spectrometry （GC/MS）. Peripheral blood lymphocyte DNA damage was analyzed by comet assay. The relationship between serum MTBE content and lymphocyte DNA damage was explored using Pearson correlation analysis. Results ] All selected workers met the basic health requirements for gasoline station operators. The mean value of MTBE in the exposure group was （6.230 ± 2.369） μg/L, significantly higher than that in the control group, （5.164 ± 2.139） μg/L （P 〈 0.05）. The mean value of Olive tail moment in the exposure group was （0.060 ± 0.045）μm, significantly higher than that in the control group, （0.039 ± 0.038）μm （P 〈 0.05）. The results of Pearson correlation analysis showed a positive correlation between MTBE content and the degree of DNA damage （r=0.859, P〈 0.05）. Conclusion ] There is a correlation between serum MTBE content and lymphocyte DNA damage.