慢病毒介导bFGF基因修饰的骨髓基质细胞对脑梗死后血管新生的影响

目的探讨慢病毒介导碱性成纤维细胞生长因子（basic fibrobla stgrowth factor，bFGF）基因修饰的骨髓基质细胞（bone marrow stromal cells，BMSCs）对脑梗死后血管新生的影响。方法利用慢病毒载体介导bFGF基因修饰BMSCs，获得稳定转染bFGF的BMSCs。脑梗死后1d立体定向移植至梗死灶周围。在MCAO术前及术后1、3、7、14d进行神经功能评分。术后14d股静脉注射异硫氰酸荧光素右旋糖酐（FITC-dextran）标记微血管，结合共聚焦显微镜和3DDoctor3．5版软件分析梗死灶周围区微血管的直径、面积及血管分支数目。结果bFGF-BMSCs组术后3d神经功能恢复明显优于对照组与BMSCs组（P〈0．05），BM—SCs组和bFGF-BMSCs组术后7d及14dmNSS评分显著低于对照组（P〈0．05），而且bFGF-BMSCs组神经功能恢复好于BMSCs组（P〈O．05）。bFGF-BMSCs组微血管直径、分支数目及面积显著性高于对照组（P〈0．01）和BMSCs组（P〈0．05）。结论bFGF修饰的B^假瞄能更好地促进脑梗死后神经功能恢复及血管新生。

Effect of ientiviral vector mediated bFGF gene-modified bone marrow stromal cells on angiogenesis in rats with cerebral ischemia

Objective To investigate the influence of lentiviral vector mediated basic fibmblast growth factor （bFGF） gene modified bone marrow stromal cells （BMSCs） on angiogenesis in rats with cerebral ischemi＆ Methods Rat BMSCs were lentivirally tranduced to express bFGF stably. They were transplanted into the ischemic boundary zone 1 d after permanent middle cerebral artery occlusion. The neurological score were evaluated by modified neurolog- ical severity score （mNSS） before and ld, 3 d, 7 d and 14 d after MCAO. Microvessel diameters, numbers of branch points and vessel areas were analyzed in cerebral microvessels at the isehemic boundary perfused with fluorescein iso- thiocyanate （FITC） dextran on the 14th day after ischemia using laser scanning confocal microscopy （LSCM） and 3 D Doctor 3. 5 software Results Compared with control group and BMSCs group, bFGF-BM~s group exhibited more significant recovery 3 d after MCAO （P~0. 05） . The mNSS were significantly decreased in BMSCs group and bF- GF-BMS~;s group compared to control group 7 d and 14 d after MCAO （P〈0. 05）. And recovery was better in bF- GF-BM~s group than in B~s group （P~0. 05）. The microvessel diameters, the number of vascular branch points and the vessel areas were significantly increased in bFGF-BM~s group as compared to control group （P〈 0. 01 ） and B~s group （P〈0. 05）. Conclusions bFGF gene-modified B~s can better improve neurological re- covery and angiogenesis after stroke