IL-13对正常人表皮成纤维细胞炎症及纤维化相关基因表达的影响

目的探讨IL-13对正常人表皮成纤维细胞(NHDF)炎症及纤维化相关基因的作用,从而进一步了解IL-13在皮肤纤维化过程中的病理机制。方法将不同浓度的IL-13(0.1ng/mL,1ng/mL,10ng/mL)加入到体外培养的NHDF,作用24h后,采用实时定量PCR(Real-TimePCR)方法 ,研究不同浓度IL-13刺激NHDF后CCL-2,IL-13ra1,IL-13ra2,CTGF和Pro-collagenI基因的表达情况。结果不同浓度的IL-13刺激NHDF24h后,CCL-2,IL-13ra2和Pro-collagenI基因表达显著增加(P0.05),并呈剂量依赖关系;IL-13ra1和CTGF基因表达无明显改变(P0.05)。结论 CCL-2,IL-13ra2和Pro-collagenI基因的高表达可能参与了IL-13在皮肤组织纤维化中的病理过程。

The Study of Inflammatory and Fibrosis-related Genes Expression on Interleukin 13 Treated Normal Human Dermal Fibroblasts

Objective To investigate the effects of IL-13 on inflammatory and fibrosis-related genes expression in normal human dermal fibroblasts(NHDF).Methods To add different concentrations of IL-13 (0.1ng/mL,1ng/mL,10ng/mL) into the in-vitro-cultured NHDF,after 24-hours stimulation,extracted RNA,and applied the Real-Time PCR to investigate the genes expression of CCL-2,IL-13ra1,IL-13ra2,CTGF and Pro-collagen I in IL-13 treated NHDF.Results After 24-hours IL-13 stimulation in NHDF,the genes expressing of CCL-2,IL-13ra2 and Pro-collagen I were significantly and dose-dependently increased (P<0.05),while the IL-13ra1 and CTGF genes expressing were not significantly increased (P>0.05).Conclusion Our results indicate that,CCL-2,IL-13ra2 and Pro-collagen I genes over expressing may involved in IL-13 pathological role in skin fibrosis.