New Activities for Isolated Compounds from Convolvulus austro‐aegyptiacus as Anti‐ulcerogenic,Anti‐Helicobacter pylori and Their Mimic Synthesis Using Bio‐guided Fractionation |
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| Authors: | Amani S. Awaad Asmaa A. Al‐Rifai Reham M. El‐Meligy Ahmed M. Alafeefy Mohamed E. Zain |
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| Affiliation: | 1. Pharmacognosy Department, College of Pharmacy, Salman bin Abdulaziz University, Al‐Kharj, KSA;2. Chemistry Department, College of Science, King Saud University, Riyadh, KSA;3. Aromatic and Medicinal Plant Dept., Desert Research Centre, Cairo, Egypt;4. Medicinal Chemistry Department, College of Pharmacy, Salman bin Abdulaziz University, Al‐Kharj, KSA;5. Botany and Microbiology Department, Faculty of Science, Al‐Azhar University, Cairo, Egypt |
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| Abstract: | Bio‐guided fractionation of the total alcoholic extract of Convolvulus austro‐aegyptiacus was screened for its anti‐ulcerogenic activity, using an absolute‐ethanol‐induced ulcer model at 500 and 1000 mg/kg doses. Two compounds were isolated from the butanol extract of C. austro‐aegyptiacus and identified by 1H and 13C nuclear magnetic resonance as scopoletin and scopolin. The isolated compounds (50 mg/kg) showed a remarkable anti‐ulcerogenic activity because they exhibited control‐ulcer protection by 16.7% and 90.8%, respectively. The acute toxicity study showed that the extract is highly safe; the median lethal dose (LD50) was more than 4000 mg/kg. Moreover, the obtained results were confirmed by the sub‐chronic toxicity because the rats that have been administered 1000 mg/kg of the extract for 15 consecutive days showed no alteration in the liver and kidney functions. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | peptic ulcer scopoletin scopolin H. pylori liver and kidney functions cytoprotective |
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